Pyrrolidine dithiocarbamate and saxagliptin ameliorate ulcerative colitis in rats
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2019, Vol 9, Issue 9
Abstract
Objective: To evaluate the antioxidant, immunomodulatory and anti-inflammatory activities of pyrrolidine dithiocarbamate and saxagliptin in rats with thioacetamide-induced ulcerative colitis. Methods: Animals were orally administered with a vehicle, sulfasalazine (500 mg/kg), pyrrolidine dithiocarbamate (100 mg/kg), and saxagliptin (10 mg/kg) for two weeks. Ulcerative colitis was induced by a single intrarectal instillation of thioacetamide on day 8. Colon samples were collected to assess mitogen-activated protein kinase (MAPK), phosphorylated extracellular signal-regulated kinase (ERK), cAMP response element-binding protein (CREB), interleukin-12 (IL-12), caspase-3, β-defensin, inducible nitric oxide synthase (iNOS) and glucagon like peptide-1 (GLP-1). Moreover, histopathological examination was performed. Results: Rats treated with thioacetamide caused increases in colonic MAPK, phosphorylated ERK, CREB, caspase-3, IL-12, β-defensin, iNOS, as well as decreases in body weight and GLP-1. In addition, distortion of colonic structure was found by histopathological examination. Pyrrolidine dithiocarbamate and saxagliptin mitigated colitis severity by improving body weight decrease and GLP-1, and reducing colonic MAPK, phosphorylated ERK, CREB, caspase-3, IL-12, β-defensin and iNOS. Conclusions: Pyrrolidine dithiocarbamate and saxagliptin are efficient against thioacetamide induced colitis through improving inflammatory and oxidative changes.
Authors and Affiliations
Berween Mahmoud Elmahmoudy, Mai A. Abd El Fattah, Mohamed F. Elyamany, Laila A. Rashed
Keywords
Related Articles
- EP ID EP640782
- DOI 10.4103/2221-1691.267638
- Views 64
- Downloads 0
The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme
Objective: To determine the effects of different strength of Murashige and Skoog (MS) media (full, 1/2 and 1/4) in solid and liquid media on in vitro growth of Typhonium flagelliforme (T. flagelliforme), whereby an optim...
Larvicidal activity of Neem oil and three plant essential oils from Senegal against Chrysodeixis chalcites (Esper, 1789)
Objective: To evaluate the insecticide, larvicidal and repellent activity of the essential oils from Callistemon viminalis, Melaleuca leucadendron, and Hyptis suaveolens against Chrysodeixis chalcites and to compare it w...
Fate of polyphenols in pili (Canarium ovatum Engl.) pomace after in vitro simulated digestion
Objective: To evaluate the stability and bioavailability of polyphenols in pili (Canarium ovatum Engl.) pomace during simulated in vitro digestion. Methods: Freeze-dried pili pomace was subjected to in vitro digestion si...
Euphorbia tirucalli L.: Review on morphology, medicinal uses, phytochemistry and pharmacological activities
The intention of current review is to make available up-to-date information on morphology, ecological biodiversity, medicinal uses, phytochemistry and pharmacological activities on different parts of Euphorbia tirucalli...
Chromatographic fingerprinting and free-radical scavenging activity of ethanol extracts of Muntingia calabura L. leaves and stems
Objective: To determine the thin-layer chromatography (TLC) fingerprint profiles and to evaluate the in vitro antioxidant activity of ethanol extracts of Muntingia calabura (M. calabura) leaves and stems. Methods: The le...