QUALITATIVE ANALYSIS OF LACTOBACILLUSSTRAINS IN MILK PROBIOTICS.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 10
Abstract
In the present study, an attempt was taken for qualitative analysis (to detect the presence) of Lactobacillusstrains: one of the major bacterial strains of milk probiotics by different biochemical tests, salt & pH tolerance and subsequently sugar & casein fermentation tests of cheese, curd and milk kefir.Gram staining and colony morphology of each isolated bacterial colony primarily indicated the presence of Lactobacillus species which was further confirmed by different biochemical and selective tests for Lactobacillus species. Negative catalase test as well as positive Kilger?s iron agar, oxidase and Simon citrate as biochemical tests confirmed the presence of Lactobacillus species in the test samples. Additionally, good to moderate sugar (sucrose, fructose, glucose & lactose) and skim milk fermentation revealing deep red color and clear zone appeared around the areas in the medium where the organism had grown, proved the presence of Lactobacillus species in the samples. Moreover, less growth or absence of bacteria in MRS broth medium containing higher salt concentration (8% NaCl solution and above) and having higher pH value by adding 1N HCl (pH 3 and above) finally confirmed that cheese, curd and milk kefircontain Lactobacillus species.
Authors and Affiliations
Md. Hamiduzzaman, Fayad Bin Abdus Salam, A. H. M. Nazmul Hasan, Sayma Afroz Santa.
Keywords
Related Articles
- EP ID EP409460
- DOI 10.21474/IJAR01/7795
- Views 59
- Downloads 0
POLICY OF GOVERNMENT OF THE REPUBLIC OF INDONESIA IN THE PROTECTION AND CONSERVATION OF REGIONAL LANGUAGES: A LANGUAGE PLANNING STUDY.
This study analyzes the policies both at the national level, as well as the regional level that has been applied by the Indonesian government in protecting the regional languages in Indonesia. This research uses descript...
IN VIVO PROTECTIVE EFFECT OF NELSONIA CANESCENS (LAM.) SPRENG EXTRACTS AGAINST CARBON TETRACHLORIDE-INDUCED HEPATOTOXICITY IN RATS.
Objective: To investigate the in vivo protective effect of Nelsoniacanescens extracts against carbon tetrachloride-induced hepatotoxicity in rats. Methods: The anti-hepatotoxicity activity was assessed through toxicity s...
THE IMPACT OF ORGANIZATIONAL CULTURE ON ADOPTING THE AGILE METHOD IN SERVICE PROJECTS.
The article will address one subject that most researchers hesitate or avoid tackling. Criticizing organizational culture and assessing its impact on important decisions is not a popular endeavor. The article highlights...
ATTENUATION OF HEMODYNAMIC RESPONSES TO ENDOTRACHEAL INTUBATION: COMPARISON OF CLONIDINE AND ESMOLOL.
Objective: The objective of the study was to compare the effects of esmolol with those of clonidine on the prevention of tachycardia and hypertension caused by endotracheal intubation. Methods: This was a prospective, ra...
USAGE OF INTRAMUSCULAR METHOTREXATE IN THE SUCCESSFUL MANAGEMENT OF CAESAREAN SCAR PREGNANCY: A RARE CASE REPORT.
Caesarean scar pregnancy is the rarest type of ectopic pregnancy, associated with a high risk of morbidities and complications, such as uterine disruption and extensive hemorrhage [1]. The incidence of scar pregnancies i...