Qualitative and Quantitative Analysis of Phylloplane Mycoflora of Stevia Rebaudiana Bertoni
Journal Title: JOURNAL OF BIOLOGICAL ENGINEERING RESEARCH AND REVIEW - Year 2019, Vol 6, Issue 1
Abstract
The aim of the present investigation is to study the fungal flora of young and mature leaves of S. rebaudiana. Young and mature leaves of S. rebaudiana were used for isolation of fungi. Phylloplane mycoflora were isolated from leaves of S. rebaudiana by using three methods i.e. dilution plate method, leaf impression method and leaf washing method. Dilution plate method and leaf washing method were applied for quantitative analysis and leaf impression method for qualitative analysis. A total of 35 fungal species comprising Zygomycetes, Ascomycetes and Deuteromycetes including sterile forms were isolated during the course of investigation. A total of thirteen fungal species viz. Aspergillus niger, A. flavus, A. fumigatus, Penicillium cyclopium, P. chrysogenum, Cladosporium elatum, C. cladosporioides, Mucor etc. were isolated from mature leaves. Eleven fungal species were found by leaf impression method, eight by leaf washing method and six by dilution plate method. Aspergillus, Penicillium and Cladosporium species were found to be dominant among all the species. Phylloplane fungal population on mature leaves of S. rebaudiana was 1.68×102 /cm2 leaf area. Thus, the outcome of study presents a clear picture about fungal population and diversity in phylloplane of S. rebaudiana mature leaves.
Authors and Affiliations
Reshu Chauhan, Navneet Navneet
Keywords
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