Abstract

Objective: To justify the folkloric uses of Sesbania grandiflora (S. grandiflora) leaves, 90% ethanolic extract and their organic and aqueous partitioning substances were evaluated for their possible antioxidant, antidiarrhoeal and antimicrobial activity in vivo. Methods: Crude ethanolic extract of S. grandiflora leaves were being subjected to partitioned into different fractions using solvents of different polarity. Antioxidant, antimicrobial, and antidiarrheal activity was evaluated and subsequently results were corresponded with the conventional standard drugs. Results: The antidiarrheal activity was assessed using mouse model, where unfractionated crude ethanolic extract showed 25.0% at a dose of 200 mg/kg and 38.1% at dose 400 mg/ kg body weight and the reference drug loperamide 53.6% at a dose of 50 mg/kg inhibited diarrhoeal episode. Crude ethanolic extract and extract fractions significantly inhibit the growth of known bacteria cause urinary tract infection. The total phenolic and flavonoid content was found maximum in chloroform soluble fractions (39.47 ± 0.33) and (31.44 ± 0.47) respectively. Extractives showed satisfactory antioxidant potential in DPPH free radical scavenging assay with IC 50 values found in petroleum ether soluble fractions (18.05 ± 1.90) in comparison with ascorbic acid (17.50 ± 0.20). Conclusions: Ethanolic extract and their aqueous and organic fractious revealed the leaves of S. grandiflora have the potential remedy of diarrhea, known bacteria cause urinary tract infection and potent antioxidant activity which ensure the folkloric use of the leaves of grandiflora.

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