Qualitative ultrastructural changes and morphometry of deccani sheep spermatozoa preserved with egg yolk citrate extender
Journal Title: Journal of Applied and Natural Science - Year 2017, Vol 9, Issue 3
Abstract
The present investigation was aimed to study the sequential changes in the sperm cell deterioration dur-ing liquid storage of Deccani sheep breed semen from dilution to 48 h of storage along with its seminal characteris-tics and sperm morphometric measurement. Thus the two Deccani adult rams (aged 2 years), were selected (six ejaculates/each ram) and the collected semen was diluted with Egg yolk citrate extender (EYC) (final concentration - 400 million spermatozoa/0.2 ml semen).Seminal characteristics were assessed along with sperm morphologi-cal changes by Electron microscopy immediately after dilution, at 24 and 48 h of storage, respectively. Sperm morphometry was analysed by Image analysis.The percentage of Individual motility, Live spermatozoa, Acrosomal integrity and Hos-test reactive sperm decreased significantly (P<0.05) from 80.41 to 49.16%, 82.75 to 51.25%, 94.16 to 83% and 76 to 48.58%, respectively during liquid storage of semen from initial dilution to 48 h of storage. The sperm head length (μm), Head width, sperm head area (μm2), sperm head perimeter (μm), mid piece length (μm), proximal mid piece width (μm), distal midpiece width (μm), volume of mid piece (μm3) and acrosomal cap length (μm) were 7.80, 4.33, 26.84, 20.63, 14.03, 0.74, 0.51, 4.54 and 5.24, respectively. Electron microscopic qualitative evaluation revealed that the main site of injury is the apical ridge of ram spermatozoa when stored at 5ºC. The electron density of the mitochondria reduced indicating concomittant depletion of ATP and loss of motility resulting in reduction of fertility.
Authors and Affiliations
Madishetti Rajashri, Komati Ramchandra Reddy, Gangineni Aruna Kumari, Nagireddy Nalini Kumari, Surabhi Kesharwani, Gandham Srinivas
Keywords
Related Articles
- EP ID EP286748
- DOI 10.31018/jans.v9i3.1394
- Views 61
- Downloads 0
Pongamia pinnata L. (Karanja) based agri-silviculture system under rainfed conditions of south-west Haryana
The present study was conducted to assess the the performance of Karanja based agri-silviculture system in rainfed conditions of south west Haryana. The results showed that the yield (grain and fodder) of different crops...
Genome fingerprinting of two populations of Aedes (Stegomyia) albopictus Skuse (Asian Tiger) using random primers (Diptera: Culicidae)
The present paper deals with RAPD-PCR based genomic characterization of two allopatric populations of Aedes (Stegomyia) albopictus Skuse which is a major vector of dengue fever and a source of many other pathogenic infec...
Mungbean yield and nutrient uptake performance in response of NPK and lime levels under acid soil in Vindhyan region, India
2016A field experiment was conducted to understanding the management of soil acidity with NPK and lime levels for sustainable mungbean productivity, Crop was sown during kharif season of 2014at Agronomy farm of Rajiv Gan...
Signature capture of red soil patches and their acidity-A case study of Banka district, Bihar, India
Colour in soils as well as other object is the visual perceptual property which is perceived by human eye. They are governed by spectrum of light corresponding to wavelength or reflected energy of the material. Developed...
Histopathological changes in the gill architecture of Labeo rohita from the pond adjacent to thermal power station, Koradi, Nagpur, India
The hazardous effect of water pollution on the histopathology of selected organs of the freshwater fish Labeo rohita was studied with respect to thermal power plant effluent. The purpose of this research paper is to stud...