QUANTIFICATION OF EXEMESTANE ACCUMULATION DURING MICROBIAL BIOCONVERSION BY TLC IMAGE ANALYSIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 8
Abstract
Objective: The present study was aimed at developing a rapid, cost effective and accurate method for quantification of exemestane using thin layer chromatography (TLC) separation followed by image analysis and to test it for monitoring the accumulation of exemestane during microbial bioconversion.Methods: After microbial bioconversion and TLC separation of products formed, exemestane was quantified using ImageQuant TL v2003 image analysis software and the results were compared with high performance liquid chromatography (HPLC) analysis.Results: The percentage error between TLC and HPLC analyses was ranged from (-) 5.18 to (+) 5.51. Bacterial strains Arthrobacter simplex IAM 1660, Nocardia sp. MTCC 1534, Pseudomonas putida MTCC 1194 and Rhodococcus rhodochorus MTCC 291 respectively yielded 79.7 (72 h), 63.9 (72 h), 69.8 (96 h) and 83.2 (96 h) mole percent bioconversion of 6-methylene androstenedione to exemestane. Conclusion: Rhodococcus rhodochorus MTCC 291 was found to be the most suitable organism for the bioconversion and may be used to develop an eco-friendly route to replace chemical synthesis that eliminates the use of toxic chemicals and side products.
Authors and Affiliations
Prachi Patil, Rajesh Sharma, Tushar Banerjee, Shridhar Patil
Keywords
Related Articles
- EP ID EP575561
- DOI 10.22159/ijpps.2017v9i8.19201
- Views 109
- Downloads 0
HEPATOPROTECTIVE ACTIVITY OF MONASCUS PURPUREUS (RED RICE YEAST) IN DIABETIC RATS ALONE OR IN COMBINATION WITH PIOGLITAZONE: AN EFFECT MEDIATED THROUGH CYTOKINES, ANTIOXIDANTS AND LIPID BIOMARKERS
Objective: Diabetes induces many complications such as cardiovascular problems, cataracts, kidney damage and polyneuropathy. Streptozotocin (STZ) induced diabetes is considered one of the most common animal models in rat...
QUALITY MANAGEMENT SYSTEM IN CHANGE CONTROL AT INDUSTRY LEVEL: AN OVERVIEW
Change is inevitable in a pharmaceutical manufacturing operation. Vendors change processes, sources, and specifications for raw materials, equipment requires repair, service, or replacement, manufacturing locations are c...
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IMPURITIES FROM OLMESARTAN MEDOXIMIL AND HYDROCHLOROTHIAZIDE TABLET
Objective: To develop and validate stability indicating RP-HPLC gradient method for simultaneous estimation of impurities and degradation products from Olmesartan Medoximil and Hydrochlorothiazide tablet.Methods: The chr...
CHEMICAL COMPOSITION AND ANTIFUNGAL ACTIVITY OF ESSENTIAL OIL FROM SATUREJA CALAMINTHA NEPETA AGAINST PHYTOPATHOGENS FUNGI
Objective: The increasing incidence of resistance among pathogen towards synthetic fungicides is a cause of serious problem. For this, the fight against fungi is directed to the use of natural products. The main objectiv...
THE EFFECT OF TIME INFLUENCE ON PHYSIOLOGICAL PARAMETERS FOLLOWING KETAMINE AND DIAZEPAM ADMINISTERATION IN CATS
Objective: The present study aims to determine the effect of time influence on rectal temperature, respiratory and pulse rate, onset and duration of action, duration of recumbency and recovery following ketamine and diaz...