QUANTITATIVE ANALYSIS OF PAMABROM AND IBUPROFEN IN SYNTHETIC MIXTURE USING 1ST ORDER DERIVATIVE SPECTROSCOPY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 10
Abstract
Objective: The preliminary goal was to develop and validate 1st order derivative spectroscopic method for quantitative analysis of Pamabrom (PAMA) which is a xanthine diuretic and ibuprofen (IBU) which is a non-steroidal anti-inflammatory agent from its synthetic mixture. Methods: Analytical method was developed on Shimadzu double beam spectrophotometer equipped with UV probe 2.42 as software using methanol as solvent. Quantification of PAMA was carried out at zero cross over point of IBU that is 291 nm and for IBU, it was achieved at 278 nm which is zero cross over point of PAMA. Method was validated according to ICH Q2 R1 guidelines. Results: Method showed a linear response in the range of 2-12 µg/ml of PAMA and 20-120 µg/ml of IBU. Method was found to be accurate with recovery between 99.7–100.9 % for PAMA and 100.3–100.7 % for IBU. The method was found to be accurate and precise for quantitative analysis of PAMA and IBU. Conclusion: The developed method was successfully validated as per ICH Q2 R1 guidelines and was successfully applied for quantitative analysis of a synthetic mixture of PAMA and IBU.
Authors and Affiliations
PINAK PATEL, PRIYANKA VASANI, BINNY MEHTA, KRUNAL DETHOLIA
Keywords
Related Articles
- EP ID EP646240
- DOI 10.22159/ijpps.2019v11i10.34797
- Views 93
- Downloads 0
ANTIDIABETIC ACTIVITY OF SEED OF EUGENIA JAMBOLANA IN STREPTOZOTOCIN INDUCED DIABETIC MALE ALBINO RAT: AN APOPTOTIC AND GENOMIC APPROACH
Objective: The study aimed to investigate the antidiabetic effect of ethyl acetate fraction of seed of Eugenia jambolana (E. jambolana) at genomic level in streptozotocin (STZ) induced diabetic male albino rat.Methods: D...
A BIOGENIC APPROACH FOR THE SYNTHESIS AND CHARACTERIZATION OF ZINC OXIDE NANOPARTICLES PRODUCED BY TINOSPORA CORDIFOLIA
Objective: The study mainly focuses on the preparation and characterization of Zinc oxide nanoparticles using an aqueous stem extract of Tinospora cordifolia.Methods: Zinc Oxide nanoparticles were synthesized by a biolog...
HEPATOPROTECTIVE ACTIVITY OF SPHAERANTHUS AMARANTHOIDES WHOLE PLANT IN PARACETAMOL INDUCED HEPATOTOXICITY IN RATS
Objective: To evaluate the protective effect of ethanol extract of whole plant of Sphaeranthus amaranthoides against paracetamol induced hepatotoxicity in albino wistar rats.Methods: Liver damage was induced by administr...
EFFECT OF CHLOROPHYLLIN, AN SEMI-SYNTHETIC CHLOROPHYLL MOLECULE ON HYPERGLYCEMIA AND HYPERLIPIDEMIA IN STREPTOZOTOCIN INDUCED DIABETIC MICE
Objective: The aim of the present study was to determine the effect of chlorophyllin (CHL) on hyperglycemia and hyperlipidemia in streptozotocin (STZ) induced diabetic mice.Methods: Diabetes was induced in mice by admini...
COMPARATIVE STUDIES OF ANTIOXIDANT ACTIVITY FROM THE PETALS OF SELECTED INDIAN MEDICINAL PLANTS
Objective: The present study was to evaluate the in vitro antioxidant activity from the petals of four different Indian medicinal plants (Punica granatum, Hibiscus rosasinensis, Cassia auriculata and Moringa oleifera).Me...