Quantitative Estimation of Four Sartans in Presence of Hydrochlorothiazide in Pharmaceutical Preparations by High Performance Liquid Chromatography
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 11, Issue 4
Abstract
A simple, reliable, reproducible, economical and rapid isocratic RP-HPLC method was developed for the simultaneously separated and quantification of four angiotensin II-receptor antagonists namely Telmisartan (TELM), Losartan (LOSA), Olmesartan (OLME) and Valsartan (VALS) along with thiazide diuretic mostly Hydrochlorothiazide (HCTZ). All the above said drugs were separated by using Welchrom C18 column having internal diameter of 4.6 X 250 mm with 5 µm particle size as stationary phase with mobile phase consisting a mixture of acetonitrile and phosphate buffer (pH-3.3, 50:50 v/v). The mobile phase was pumped at a flow rate of 1 ml/min. The wave length of the UV detection was done at 238 nm. All the sartans were separated within 6 minutes. The calibration curves were linear (r2 = 0.9998) in all cases. The relative standard deviation was less than 2% and average recovery was above 99.95%. All four sartans were routinely assayed without interference. This method was statistically validated in different parameters like linearity, precision, specificity, accuracy, robustness and ruggedness. The optimized method proved to be accurate, specific and robust for the quality control of four angiotensin-II receptor blockers alone or in combination with HCTZ in bulk drug and tablet dosage forms.
Authors and Affiliations
Panchumarthy Ravisankar, Kurra Manjusha, V. Laya Sri, B. Vijaya Kumar, K. Rajyalakshmi, P. Srinivasa Babu
Keywords
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- EP ID EP342265
- DOI 10.9734/BJPR/2016/25354
- Views 76
- Downloads 0
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