Quantitative Estimation of Four Sartans in Presence of Hydrochlorothiazide in Pharmaceutical Preparations by High Performance Liquid Chromatography
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 11, Issue 4
Abstract
A simple, reliable, reproducible, economical and rapid isocratic RP-HPLC method was developed for the simultaneously separated and quantification of four angiotensin II-receptor antagonists namely Telmisartan (TELM), Losartan (LOSA), Olmesartan (OLME) and Valsartan (VALS) along with thiazide diuretic mostly Hydrochlorothiazide (HCTZ). All the above said drugs were separated by using Welchrom C18 column having internal diameter of 4.6 X 250 mm with 5 µm particle size as stationary phase with mobile phase consisting a mixture of acetonitrile and phosphate buffer (pH-3.3, 50:50 v/v). The mobile phase was pumped at a flow rate of 1 ml/min. The wave length of the UV detection was done at 238 nm. All the sartans were separated within 6 minutes. The calibration curves were linear (r2 = 0.9998) in all cases. The relative standard deviation was less than 2% and average recovery was above 99.95%. All four sartans were routinely assayed without interference. This method was statistically validated in different parameters like linearity, precision, specificity, accuracy, robustness and ruggedness. The optimized method proved to be accurate, specific and robust for the quality control of four angiotensin-II receptor blockers alone or in combination with HCTZ in bulk drug and tablet dosage forms.
Authors and Affiliations
Panchumarthy Ravisankar, Kurra Manjusha, V. Laya Sri, B. Vijaya Kumar, K. Rajyalakshmi, P. Srinivasa Babu
Keywords
Related Articles
- EP ID EP342265
- DOI 10.9734/BJPR/2016/25354
- Views 63
- Downloads 0
Development and Evaluation of Ophthalmic Drop and In-situ Gel from Roots of Boerhaavia diffusa
The present investigation deals to evaluate efficacy of ophthalmic drop and in-situ gel formulations of aqueous distillate of Boerhaavia diffusa roots using polymers sodium alginate (SA), HPMC (hydroxyl propyl methyl cel...
Review of Traditionally Used Medicinal Plants by the Kipsigis Community in Kenya
Background: Every community in Kenya have their own ways of providing good health care services for its members. Such services are geared towards providing therapeutic and medical related services for the upkeep of good...
Medicinal Values of Trichosanthus cucumerina L. (Snake Gourd) - A Review
Snake gourd is an annual climber and it’s commonly called as snake gourd, viper gourd, snake tomato or long tomato. The fruit is usually consumed as a vegetable due to it is good nutritional value. The fruit is a good so...
Anxiolytic, Sedative and Hypothermic Effects of Aqueous Leaf Extract of Vernonia amygdalina Del. (Asteraceae) in Albino Mice
Aims: This study evaluated the anxiolytic, sedative and hypothermic effects of aqueous leaf extract of Vernonia amygdalina in Mice. Study Design: One-factor two control groups experimental design. Place and Duration of...
Evaluation of the Physicochemical and in vitro Dissolution Properties of Metformin Hydrochloride Tablet Brands Marketed in Five Cities in Ghana
Aim: A comparative evaluation of the physicochemical and in vitro dissolution properties of metformin hydrochloride tablet brands sampled in five cities in Ghana was undertaken to assess their pharmaceutical equivalence....