Radiation-enhanced endostatin gene expression and effects of combination treatment.
Journal Title: Technology in Cancer Research & Treatment - Year 2005, Vol 4, Issue 2
Abstract
Targeting cells that support tumor growth by administering potent angiogenesisinhibitors is currently an area of intense interest. In the present study, a unique plasmid vector forthe mouse endostatin gene, pXLG-mEndo, was constructed and evaluated with and without radiation usingthe Lewis lung carcinoma (LLC) cell line. The physical properties of the expressed endostatin proteinwere validated by PCR, gel electrophoresis, and Western blot. Enzyme-linked immunosorbent and immunocytochemicalanalyses for the therapeutic gene demonstrated that transfected LLC cells secreted the protein into themedium. Exposure of the cells to 2 gray (Gy) gamma-rays reduced the time to reach the maximum expressionlevel of the endostatin gene and also increased the amount of secreted endostatin protein (P
Authors and Affiliations
Xian Luo, Melba L Andres, Tatyana M Timiryasova, Istvan Fodor, James M Slater, Daila S Gridley
Keywords
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- EP ID EP83762
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Radiation-enhanced endostatin gene expression and effects of combination treatment.
Targeting cells that support tumor growth by administering potent angiogenesisinhibitors is currently an area of intense interest. In the present study, a unique plasmid vector forthe mouse endostatin gene, pXLG-mEndo, w...