RADICAL SCAVENGING ACTIVITY OF TRITERPENE STEROIDS FROM STEM OFPOLYGONUM PULCHRUM Bl
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: P. pulchrum grows abundantly in Kendari (Sulawesi Tenggara province, Indonesia).However, there is no report neither chemical contents nor biological activitiesof the plant. This project studiesthe isolation, structure elucidations, and radical scavenging activity evaluation of triterpene steroids from stems of P. pulchrum.Methods: The isolation of the compounds was carried out by using chromatography method, i.e., vacuum liquid chromatography (VLC) and radial chromatography (RC) with silica gel as an adsorbent and various solvents as eluent. The compound structures were evaluated by spectroscopic data (FTIR and NMR data) and then the results were compared with the existing data from references. The antioxidant activity of these compounds was evaluated towards DPPH (1,1-diphenyl 2-picryl-hydrazyl).Results: Four triterpene steroids; namely, (1) 6β-hydroxystigmasta-4,22-dien-3-one, (2) stigmasterol,(3) stigmasta-4,22-dien-3-one, and (4) ergosterol peroxide, were isolated and identified from stems of P. pulchrum Bl. The antioxidant activities of all compounds were indicated by IC50 value of the compounds. The values of IC50(µM) of6β-hydroxystigmasta-4,22-dien-3-one, stigmasterol, Stigmasta-4,22-dien-3-on,ergosterol peroxide, and vitamine C (standard) toward DPPH were obtained at233.4 ± 0.28; 372.3 ± 0.33; 144.80 ± 0.24; 1083.1 ± 0.38; and 68.9 ± 0.12, respectively.Conclusions: We found that Stigmasta-4,22-dien-3-onewas the most active compound toward DPPH.Â
Authors and Affiliations
Idin Sahidin, Nohong . , Asrul Sani, Marianti Anggrenimanggau, Asep Sukohar, Harto Widodo, Syarulnataqain Baharum
Keywords
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