RADICAL SCAVENGING ACTIVITY OF TRITERPENE STEROIDS FROM STEM OFPOLYGONUM PULCHRUM Bl
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: P. pulchrum grows abundantly in Kendari (Sulawesi Tenggara province, Indonesia).However, there is no report neither chemical contents nor biological activitiesof the plant. This project studiesthe isolation, structure elucidations, and radical scavenging activity evaluation of triterpene steroids from stems of P. pulchrum.Methods: The isolation of the compounds was carried out by using chromatography method, i.e., vacuum liquid chromatography (VLC) and radial chromatography (RC) with silica gel as an adsorbent and various solvents as eluent. The compound structures were evaluated by spectroscopic data (FTIR and NMR data) and then the results were compared with the existing data from references. The antioxidant activity of these compounds was evaluated towards DPPH (1,1-diphenyl 2-picryl-hydrazyl).Results: Four triterpene steroids; namely, (1) 6β-hydroxystigmasta-4,22-dien-3-one, (2) stigmasterol,(3) stigmasta-4,22-dien-3-one, and (4) ergosterol peroxide, were isolated and identified from stems of P. pulchrum Bl. The antioxidant activities of all compounds were indicated by IC50 value of the compounds. The values of IC50(µM) of6β-hydroxystigmasta-4,22-dien-3-one, stigmasterol, Stigmasta-4,22-dien-3-on,ergosterol peroxide, and vitamine C (standard) toward DPPH were obtained at233.4 ± 0.28; 372.3 ± 0.33; 144.80 ± 0.24; 1083.1 ± 0.38; and 68.9 ± 0.12, respectively.Conclusions: We found that Stigmasta-4,22-dien-3-onewas the most active compound toward DPPH.Â
Authors and Affiliations
Idin Sahidin, Nohong . , Asrul Sani, Marianti Anggrenimanggau, Asep Sukohar, Harto Widodo, Syarulnataqain Baharum
Keywords
Related Articles
- EP ID EP579725
- DOI -
- Views 65
- Downloads 0
PHARMACOGNOSTICAL SCREENING AND DETERMINATION OF ANTIOXIDANT ACTIVITY OF NELUMBO NUCIFERA GAERTN ETHANOL SEED EXTRACT BY DIFFERENT IN VITRO MODELS
Objective: To perform the pharmacognostical screening and determination of antioxidant activity of Nelumbo nucifera Gaertn ethanol seed extract by different in vitro models.Methods: The different pharmacognostical parame...
ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF LANNA MEDICINAL PLANTS USED IN MAHOOG FORMULA
Objectives: Antibacterial and antioxidant activities of Lanna medicinal plants used in Mahoog formula were investigated.Methods: Dried powders of twenty five Lanna medicinal plants were extracted with ethanol using soxhl...
PROTECTIVE ROLE, IN -VITRO FREE RADICAL SCAVENGING ACTIVITIES OF ALANGIUM SALVIFOLIUM (LINN) AGAINEST CCL4 INDUCED HEPATIC DAMAGE IN RATS
Many hepatoprotective herbal preparations have been recommended in an alternative system of medicine for the treatment of hepatic disorders. There are few or no systemic studies have been done on the protective efficacy...
ANALYTICAL METHOD DEVELOPMENT FOR SIMULTANEOUS DETERMINATION OF UBIDECARENONE AND VITAMIN E ACETATE IN CAPSULE DOSAGE FORM BY HPLC
Objective: To develop and validate a new simple, accurate, precise and sensitive high performance liquid chromatographic method (HPLC) method for simultaneous estimation of ubidecarenone and vitamin E acetate in capsule...
DESIGN, IN-SILICO DOCKING AND PREDICTIVE ADME PROPERTIES OF NOVEL PYRAZOLINE DERIVATIVES WITH SELECTIVE HUMAN MAO INHIBITORY ACTIVITY
Objectives: Curcumin, a known hMAO-A (human Monoamine oxidase-A) inhibitor from Curcuma longa has never been recognized for this property due to its poor permeability and extensive metabolism. Thus, the main objective of...