Radiosensitizing effects of 2-deoxy-D-glucose and ferulic acid on mouse Ehrlich’s Ascites Carcinoma in Swiss albino mice
Journal Title: Journal of Research in Biology - Year 2012, Vol 2, Issue 5
Abstract
The objective of the present study is to evaluate the radiosensitizing activity of combination of 2-deoxy-D-glucose (2DG) and ferulic acid (FA) against Ehrlich’s Ascites Carcinoma (EAC) in Swiss albino mice. Extensive DNA damage has been observed in the cells collected from the peritoneal cavity of combined treatment group (2DG+FA+IR) than the other treatment modalities. Treatment of EAC tumor bearing mice with 2DG and FA before 8 Gy of hemi-body γ-radiation has resulted in the significant decrease of tumor volume and tumor weight compared with EAC control group. Further, combination of 2DG and FA along with radiation decreased the viability of tumor cells in the peritoneal fluid of EAC bearing mice. It has been also found that the percentage of EAC apoptotic cells in the peritoneal fluid has been significantly increased in the combined treatment group (2DG+FA+IR) compared with other treatment groups. Combined treatment of 2DG and FA activated caspase-3 and 9, when compared with the treatment given with radiation alone indicated radiosensitizing effect of this combination (2DG+FA). This has been further evidenced by the massive decrease of LDH activity in the 2DG+FA+IR group. On other hand, 2DG and FA combination protects the hematological changes occurred during radiation treatment. Histopathological examinations showed that the combination of 2DG and FA offers protection to normal tissues during radiation treatment. Taken together, the results of the study clearly suggested 2DG and FA combination sensitizes EAC bearing mice to radiation effects at the same time offers protection to normal tissues from radiation-induced damage
Authors and Affiliations
Bandugula Venkata Reddy, Nagarajan Rajendra Prasad
Keywords
Related Articles
- EP ID EP566018
- DOI -
- Views 82
- Downloads 0
Comparison relative of abundance of Capoeta capoeta gracilis in five streams of Gorganroud River Basin, Golestan Province, Northern Iran
In this study 1705 individuals have sampled during spawning season in five streams of Gorganroud basin and investigated the lengths and relative abundance. The length varied from 4.1-23.2 cm.there were significant differ...
Prevalence of Armigeres obturbans in the rural areas around Rajkot city, Gujarat, India
The intensive study was conuducted for the prevalence and distribution of Armigeres obturbans for the first time in the rural area surrounding Rajkot City. Seven villages were identified for the study species identificat...
Length-Weight relationship and condition factor of Channa urantimaculata (Musikasinthorn, 2000) studied in a riparian wetland of Dhemaji District, Assam, India
Present study reports the length-weight relationship, condition factor and relative condition factor of Channa aurantimaculata (Musikasinthorn, 2000), a hole dwelling snakehead endemic fish species (Goswami et al., 2006,...
Ethnobotanical study of medicinal plants in the region El Hajeb (central Morocco)
As a part of enhancement of the natural heritage of the region Meknes-Tafilalet, an ethnobotanical study was conducted in the El Hajeb region. This study describes the different uses of medicinal plants by the local popu...
Avifaunal diversity of Koradi Lake in Nagpur District of central India
Birds, the feathered bipeds have immense ecological value and serve as the bio indicators of ecosystem health. Present study deals with the study on the avifaunal diversity of Koradi lake (Lat.- 21°15’0N; Longi.- 79°5’60...