Rapid Bispecific Antibodies Based Homogeneous Immunoassay for Detection of Prostate-Specific Antigen (PSA)
Journal Title: International Journal of Biomedical and Clinical Analysis - Year 2021, Vol 1, Issue 1
Abstract
Development of rapid and economical method for detection of prostate- specific antigen (PSA) in human blood. Methods: The usual procedure for the detection of prostate cancer markers in human is prostate-specific antigen (PSA) in blood (normal level ≤ 4 ng/mL) using heterogeneous immunoassay enzyme linked immunosorbent assay (ELISA). However, a rapid homogeneous immunoassay for the detection of PSA in serum, based on bispecific antibodies, is more convenient due to its speed, accuracy and obviating the need of multiple washing steps. The assay using bispecific antibody P57 (against PSA and peroxidase) and monospecific antibody B87 (against PSA) conjugated with glucose oxidase was developed in the presence of excess catalase. Similarly, in solid phase homogeneous immunoassay the monospecific antibody B87 (against PSA) and glucose oxidase were immobilized onto a solid support (plastic) and other reagents, bio-chemicals, and bispecific antibody P57 were taken in homogeneous solution. All variables, viz., glucose oxidase, peroxidase and catalase were optimized at different PSA concentrations. Results: Homogeneous immunoassay (HIA) showed linearity of PSA detection 1-10 ng/mL whereas, solid phase homogeneous immunoassay (SPHIA) showed in the range of 1-50 ng/mL suggesting SPHIA has a broader operating range, thus much better than HIA. Detection of PSA in a homogeneous solution can be completed in 90 minutes without involving any washing and incubation steps. Conclusions: Homogeneous assay is a rapid, economical method that eliminates all washing and incubation steps of conventional ELISA.
Authors and Affiliations
Ashok Kumar, Pravin K. Bhatnagar
Keywords
Related Articles
- EP ID EP724310
- DOI https://doi.org/10.61797/ijbca.v1i1.49
- Views 76
- Downloads 0
Laboratory Medicine Workforce Shortage: Informed Public and Skilled High School Students can Fill the Gap
We have known for years that laboratory medicine would not have enough employees at all levels to effectively support the growing needs of medical doctors. According to the US Bureau of Labor Statistics prediction “MLS/M...
Chronic Obstructive Pulmonary Disease (COPD): Making Sense of Existing GWAS Findings in Indian Context
To date, more than 1456 associations have been identified for Chronic Obstructive Pulmonary Disease (COPD) risk through Genome-Wide Association Studies (GWAS). However, target genes for COPD susceptibility in the Indian...
Ιnternists’ and Endocrinologists’ Knowledge and Attitudes Regarding Periodontal Disease, Caries, Xerostomia and Diabetes Mellitus: A National Survey
Objectives: The present study aims to investigate the current knowledge, attitudes and behaviors of clinicians dealing with diabetes regarding the relationship of Diabetes Mellitus (DM) and odostomatological diseases, su...
An Unusual Case of Tuberculous Lymphadenitis and Comparison of Various Methods for Diagnosis of Tuberculous Lymphadenitis
Although tuberculous lymphadenitis (TBLN) is extremely common in India, sometimes it may pose a diagnostic dilemma even after multimodality testing. This case report is about a patient who had undergone multiple fine nee...
Tweaking Internal Quality Controls for Cost Cutting in Resource Poor Settings
Commercial Internal Quality Control (IQC) materials are used regularly nowadays by most laboratories for ensuring that their test results are accurate, and the equipment used for analysis is functioning optimally. IQC ma...