Rapid Detection Of Bovine Herpes Virus Type 1 (Bohv-1) In Egyptian Dromedary Camels
Journal Title: Journal of Applied Veterinary Sciences - Year 2020, Vol 5, Issue 2
Abstract
Bovine herpesvirus 1 (BoHV-1) is known to cause reproductive disorders in Sudanese camels. Egypt imports about 90% of its camels from Sudan, and the rest from Somalia. The BoHV1 is a viral disease of bovines that can be transmitted to camel, sheep, and goat. Due to the absence of anti-camel conjugated with fluorescein isothiocyanate (FITC) in the market, we used protein-A conjugated with FITC which binds to the Fc region of IgG of many animal species. We, therefore, prepared rabbit anti-camel IgG conjugated with FITC and compared it with protein-A conjugated with FITC to the specificity and sensitivity of these compounds in IBR detection from 35 nasal swaps in imported Egyptian dromedary camels. The sensitivity and specificity of the prepared anti-camel IgG FITC and protein-A FITC were compared using Virus Neutralization Test. The labeled protein concentration in the prepared anti-camel conjugate was 2 mg/ml which was considered as an acceptable value. The degree of labeled protein (DOL) was 5.74 cm–1M–1 and optimal DOL usually fell between 2 and 10. The titer of the prepared anti-camel IgG-FITC was 3,125. The prepared anticamel IgG–FITC and protein-A-FITC showed a sensitivity of 93.75 and 90.9%, and a specificity of 71.43% and 62.5%, respectively. Our findings show no significant difference between protein-A conjugated FITC and prepared anti-camel IgG-conjugated FITC in the rapid diagnosis of BoHV-1 in Egyptian dromedary camels.
Authors and Affiliations
Ramzy H. Hamouda, Marwa F. Mahmoud
Keywords
Related Articles
- EP ID EP680597
- DOI https://dx.doi.org/10.21608/javs.2020.85587
- Views 243
- Downloads 1
Seroprevalence of toxoplasma gondii and brucella abortus in dairy animals from the Sudan: special emphasis to their serological co-existence
Toxoplasmosis and brucellosis are the most common zoonotic food borne diseases worldwide. The abortion caused by these agents is one of the major causes of socio-economic losses in human and livestock. We thus conducted...
The Effect of Topically Applied Hyaluronic Acid on Skin Wound Healing in Rabbits
This study aims to evaluate the effect of the topical application of hyaluronic acid cream 0.2% in the skin wound healing in rabbits. Thirty two healthy New Zealand adult male rabbits approximately at the same age and we...
Preparation And Evaluation Of A Recent Infectious Laryngotracheitis (Ilt) Vaccine From A Local Field Isolate
Infectious laryngotracheitis (ILT) is a respiratory tract disease affecting chickens worldwide. The disease leads to severe production losses due to increased mortality, decreased egg production, and delayed body weight...
Evaluation of Antibiotic Resistance Pattern of Escherichia Coli and Salmonella Species Isolated from Cloaca of Indigenous Chickens in Live-bird Markets in Marodi Jeh Region, Somalia
Poultry is an important source of protein globally, today, but Escherichia coli (E. coli)and Salmonella speciescontinue to be food-borne pathogens and contribute to the growing resistance to the antimicrobial agents. The...
Isolation and Identification of the most Common Bacteria Isolated from Intestine of Broiler Chickens in Egypt
Bacteria are seriously affecting broiler chickens and the poultry industry in many countries, as well as in Egypt. Both pathogenic and nonpathogenic bacterial species have been reported in broiler chickens. The present s...