Rapid Detection of Mycobacteria using automated Detection/ Incubation System in Exudative Pleural Effusion in Alexandria Main University Hospital
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 2
Abstract
Studies have been targeting to evaluate newer automated culture systems techniques for different types of samples to provide a more rapid diagnosis relying on sensitive and specific principle with the ability to discriminate between viable and dead mycobacterial bacilli. This study aimed at assessment of bactalert, biomerieux ® culture system as a rapid diagnostic tool for tuberculous pleural effusion fluid in comparison with Lowenstein Jensen conventional culture and Zeil Nelseen stained smears. Pleural aspirate collected from 60 patients presented with exudative pleural effusion was centrifuges and the deposit divided to be inoculated on bactalert bottles, on Lowenstein Jensen media and the third part was fixed on a slide and stained with Zeil Nelseen stain. Our study confirmed previous reports that bact Alert system has very high sensitivity still with lower specificity. This finding might consider Bact Alert system a reliable, easy, rapid screening test for tuberculous pleurisy that needs confirmation via other laboratory assays.
Authors and Affiliations
Ahmed Youssef Shaaban, Nesrine Fathi Hanafi
Keywords
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- EP ID EP568255
- DOI 10.20546/ijcmas.2017.602.010
- Views 64
- Downloads 0
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