Rapid Detection of Specific Gram-negative Microorganisms Causing Bloodstream Infections in Children with the Microarray Method
Journal Title: The Journal of Pediatric Research - Year 2020, Vol 7, Issue 2
Abstract
Aim: Bloodstream infections are serious conditions that can cause significant morbidity and mortality. Early recognition of the bacteria and targeted early treatment play an important role in prognosis. The Verigene Gram-negative blood culture nucleic acid test (BC-GN; Nanosphere, USA) is a new method that can accurately identify both Gram-negative bacteria and resistance genes within 2 hours. In this study, we aimed to determine the accuracy of this assay for the rapid identification of certain Gram-negative microorganisms in children with bloodstream infections and to compare with the conventional culture method. Materials and Methods: A total of 30 patients (<18 years-old) (followed up in the Department of Pediatrics of Ege University Faculty of Medicine between December 2015-June 2016) with bloodstream infections due to Gram-negative bacteria were prospectively included. Microarray test results were compared with conventional blood culture results. Results: Klebsiella spp. (40%) were the most common and Acinetobacter spp. (20%) were the second most commonly seen Gram-negative microorganisms detected. Accurate identification via microarray analysis was 100% (12/12) for Klebsiella spp, 100% (6/6) for Acinetobacter spp., 100% (5/5) for Escherichia coli, 80% (4/5) for Enterobacter cloacae spp., 100% (1/1) for Pseudomonas aeruginosa and 100% (1/1) for Citrobacter amalonaticus, respectively. The test was able to correctly identify 96.5% of Gram-negative microorganisms that were located in the test panel. CTX-M was the most common gene responsible for resistance. Conclusion: The Verigene Gram-negative (BC-GN) microarray test (Nanosphere) is a rapid method which can correctly identify the located Gram-negative microorganisms in the panel and give results much faster than conventional methods.
Authors and Affiliations
Gülhadiye Avcu, Mehmet Soylu, Şöhret Aydemir, Deniz Yılmaz Karapınar, Zümrüt Şahbudak Bal, Serap Aksoylar, Bülent Karapınar, Funda Özgenç, Fadıl Vardar
Keywords
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