Rapid in vitro regeneration of Geranium robertianum - a valuable medicinal herb
Journal Title: Journal of Research in Ecology - Year 2018, Vol 6, Issue 2
Abstract
The present study highlights a rapid and simple micropropagation of Geranium robertianum. Micro shoots were regenerated from shoot tips and nodal explants of in vitro seedlings directly without the intervening callus phase. In vitro shoots were successfully proliferated from those explants of G. robertianum cultured on MS medium fortified with Naphthalene Acetic Acid (NAA) and of N6-Benzylaminopurine (BAP). Results showed that proliferated shoots from shoot tips reached the highest mean (3.715); compared with those from nodal explants (3.015). The application of an adjuvant, adenine sulphate (80 mg.L-1) in the optimal medium led to increase the mean shoot number per shoot tip of 4.60 shoots
Authors and Affiliations
Farqad Al-Dabagh, Maha Salih, Ali A. Mahdi Al-Salihy
Keywords
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