Rapid Recombinant Protein Production using a TuMV-Based Vector in Lettuce (Lactuca sativa L.)
Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 1
Abstract
ABSTRACT: In recent years, plant viral vectors have been increasingly developed as a successful biotechnological tool to express a wide range of foreign proteins in plants. Plant viral vectors are beneficial because of the autonomous replication and high levels of gene expression in a short time. Here, we examined the utilization of an infectious turnip mosaic virus (TuMV)-derived vector for the transient expression of the jellyfish green florescent protein (GFP) in lettuce plants. After transfection by carborandum on lettuce leaves, GFP expression was detected using florescence microscopy at 14 days post inoculation. Further analysis of GFP expression using RT-PCR and ELISA assays confirmed that gfp gene was correctly transcribed and translated in lettuce plants. Our results demonstrated, for the first time, that it is possible to use TuMV-based viral vectors to produce recombinant proteins in lettuce.
Authors and Affiliations
Malihe Mirzaee1, Mokhtar Jalali-Javaran1,*, Ahmad Moieni1, Sirous Zeinali2, Mahdi Behdani3, Masoud Shams-Bakhsh4 and Mostafa Mod
Keywords
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