Rapid Recombinant Protein Production using a TuMV-Based Vector in Lettuce (Lactuca sativa L.)
Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 1
Abstract
ABSTRACT: In recent years, plant viral vectors have been increasingly developed as a successful biotechnological tool to express a wide range of foreign proteins in plants. Plant viral vectors are beneficial because of the autonomous replication and high levels of gene expression in a short time. Here, we examined the utilization of an infectious turnip mosaic virus (TuMV)-derived vector for the transient expression of the jellyfish green florescent protein (GFP) in lettuce plants. After transfection by carborandum on lettuce leaves, GFP expression was detected using florescence microscopy at 14 days post inoculation. Further analysis of GFP expression using RT-PCR and ELISA assays confirmed that gfp gene was correctly transcribed and translated in lettuce plants. Our results demonstrated, for the first time, that it is possible to use TuMV-based viral vectors to produce recombinant proteins in lettuce.
Authors and Affiliations
Malihe Mirzaee1, Mokhtar Jalali-Javaran1,*, Ahmad Moieni1, Sirous Zeinali2, Mahdi Behdani3, Masoud Shams-Bakhsh4 and Mostafa Mod
Keywords
Related Articles
- EP ID EP96308
- DOI -
- Views 122
- Downloads 0
CHARACTERIZATION, ANTIBIOTIC SENSITIVITY OF A THERMOSTABLE AMYLASE PRODUCING HAEMOPHILUS HAEMOLYTICUS ISOLATED FROM UNKESHWAR HOT SPRING AND PREDICTION OF ORIGIN USING ANTIBIOTIC TARGET SITE
Suppressive properties of composts may be improved by microbial inoculation
ABSTRACT The use of suppressive composts instead of traditional fertilizers and pesticides is becoming more commonplace in modern agricultural biotechnology. In this study, we investigated the possibility of obtaining...
Effect of Nutrient Spray on Growth and Antioxidant Status of Brassica Juncea
Molecular and functional diversity of PGPR fluorescent Pseudomonas isolated from rhizosphere of rice (Oryza sativa L.)
ISOLATION AND CHARACTERIZATION OF CELLULASE PRODUCING PARACOCCUS PANTOTROPHUS FMR19 (JX012237) FROM GOAT RUMEN FLUID AND ITS EFFECTS ON pH, TEMPERATURE AND CARBON SOURCES