Rapid screening and evaluation of site saturated structural variants of lantibiotic, nukacin ISK-1
Journal Title: Bioresearch Communications - Year 2017, Vol 3, Issue 1
Abstract
Here we reports the feasibility of introducing saturation mutagenesis (NNK mutagenesis) in nukacin ISK-1 structural gene and to create a large number of structural analogues of this peptide to study the structure function relationship. Three individual residues as Lys1, Pro8 and His12 from the parent peptide were scanned where mutation frequencies were estimated to be around 70% in all cases. A rapid screening method for identification of a large number of nukacin ISK-1 variants form whole cell using a modified MALDI-TOF/MS method have also been developed. As many variants appeared multiple times, therefore after DNA sequencing it could be obtained a total of 37 nukacin ISK-1 variants were obtained for these three positions. We evaluated the potency of each variant by a modified spot on lawn method regardless of their productivity and hence concluded that Lys1 and Pro8 positions are highly variable whereas His12 position is relatively conserved to retain the antimicrobial activity
Authors and Affiliations
Keywords
Related Articles
- EP ID EP494912
- DOI -
- Views 165
- Downloads 0
Occurrence of Intestinal Parasites Among the Slum Children
A cross sectional research was undertaken in a slum area of Dhaka city, Bangladesh from March, 2015 to February, 2016, a total of 98 children were selected from this slum area between age group 0 to 15 years by random sa...
Protective association of IKAP T3214A polymorphism with asthma in a North Indian population: a case-control study
IKAP participates in activation of NF-κB and activated NF-κB is associated with over production of various inflammatory cytokines, chemokines and eotaxin genes in asthma. Genotyping of IκB kinase complex associated prote...
Co-circulation of Three Dengue Virus Serotypes in 2017 in Dhaka city: First report from Bangladesh
Dengue is a major public health problem in Bangladesh similar to many other tropical regions of the world. There are four serotypes of dengue virus and circulation of all four serotypes were reported in our neighbor coun...
Mouse homologues of hepatitis C virus human entry factors inhibit the entry of HCV pseudoparticles (HCVpp) into human hepatoma cells
Hepatitis C virus (HCV) is a growing public health concern worldwide. No vaccine preventing HCV has yet been developed due to the hindrance of research on suitable small animal models. Moreover, it was known that human C...
Isolation and Characterization of Lactic acid Bacteria from Indigenous Dairy Product and Preparation of Starter Culture by Freeze-drying
The quality of curd varies widely within different regions of Bangladesh which primarily depends on the starter cultures that are categorized in the group of lactic acid bacteria (LAB). Maintenance, preservation and use...