Rapid separation and determination of betulinic acid from a complex matrix using combination of TLC and RP-HPLC
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2012, Vol 25, Issue 2
Abstract
Hitherto, only a few studies are reported about using the combination of TLC and RP-HPLC for the separation and determination of analyte(s) from a complex matrix. The present study is aimed to develop a simple and rapid method for the separation and determination of betulinic acid from a complex matrix, extracts of Orthosiphon stamineus, using a combination of the two techniques. The samples having higher contents of the analyte and fewer interfering species were prepared using TLC. The samples were then eluted through C18 column using isocratic solvent system comprising acetonitrile, methanol and acetic acid acidified water of pH 2.8 in a ratio of 70 : 20 : 10 (v/v/v), respectively, and detection was carried out at 210 nm. The method was validated and applied successfully to quantify betulinic acid in various types of extracts of the plant. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.0005 and 0.0050 µg/ml, respectively. The method exhibited linearity in a concentration range of 0.005-100.00 µg/ml (R2= 0.9999). The recovery was found to be 97.10 - 97.60 % (RSD < 5%), whereas, intra-day and inter-days accuracy values were 97.13 - 98.67% (RSD < 5%) and 96.45 - 98.00% (RSD < 5%), respectively. The results of the present study indicate that the developed method is simple, rapid, sensitive and accurate, and may be of a value to natural product industry and researchers for the standardization of extracts containing betulinic acid in a lesser time and consuming fewer solvents.
Authors and Affiliations
Khalid Hussain, Muhammad Khan, Zhari Ismail, Amirin Sadikun
Keywords
Related Articles
- EP ID EP155930
- DOI -
- Views 102
- Downloads 0
Clinical effects of immunotherapy of DC-CIK combined with chemotherapy in treating patients with metastatic breast cancer
This study aimed to analyze the clinical effects of dendritic cell (DC) and cytokine-induced killer (CIK) immunotherapy combined with chemotherapy on patients with metastatic breast cancer. Twenty patients were inc...
Haloperidol-induced extra pyramidal symptoms attenuated by imipramine in rats
Effects of administration of imipramine (IMI) are determined on haloperidol-induced extrapyramidal symptoms (EPS). Haloperidol is administered orally at a dose of 0.2 mg/rat/day in rats for a period of 5 weeks, by...
The detection of YMDD mutation of hepatitis B virus by allele-specific PCR and a cross-contamination proofed device
Lamivudine (LAM) is commonly used to treat Hepatitis B virus (HBV) infection, but its use frequently induces drug resistance. Therefore, rapid and correct detection of drug-resistant HBV is important for effective...
Evaluation of phytoconstituents and anti-nephrotoxic and antioxidant activities of Monochoria
Monochoria vaginalis is an herbaceous medicinal plant used to treat, liver problems India. Acetaminophen is a commonly used analgesic and antipyretic agent which, at high doses, causes liver and kidney necrosis in man an...
Antidiarrhoeal, Anti-inflammatory and analgesic activities of Symplocos racemesa roxb. Bark
The antidiarrheal activity of the drug Symplocos racemosa was performed in-vivo on isolated rabbit intestine. The effects of crude extract and fractions were observed at different doses. The overall response of the...