REACTION OF EXPLANTS FROM LAVANDULA ANGUSTIFOLIA MILLER, CULTIVATED IN VITRO ON THE PURPOSE OF MULTIPLICATION AND CALLUS DIFFERENTIATION
Journal Title: Natural Resources and Sustainable Development - Year 2015, Vol 5, Issue 1
Abstract
Lavanda angustifolia Mill. is a honey, medicinal and plant ameliorative specie, which lives in arid and semi-arid climate (of mediteranean origine) and has a great echological plasticity and adjustment capacity. We studied and followed in vitro culture of apical tissue and of bud tissue cut from mature lavender plant grown on basic MS (Murashige and Skoog, 1962), more complex, with extra aminoacidglycine, with the variants: Mt = MS + 2 mg/l glycine; V1 = Mt + 1.0 mg/l AIA + 1.5 mg/l BA; V2 = Mt + 1.0 mg/l AIB + 1.5 mg/l BA; V3= Mt +1.0 mg/l ANA + 1.5 mg/l BA. The culture mediums have been conceived to prove the implication of glycine and of certain auxines in the case of in vitro cultivated lavender plants, in regeneration of plants, callus differentiations, in vitro propagation (given by the number of seedlings/explant and by their structure). After cca. 3 month of in vitro incubation, apex regenerates in 35% on Mt; 55% on V1 and 75% on V2 and forms rootless 2-3 neo-seedlings of 1-2 cm length. On V3 a large mass of callus is differentiated, of 4-5 cmØ at 85% of the explants.The bud has a better regenerating capacity (from 42%, 62% up to 80%) on V3 , callus differentiation is 85% as well,, the number of differentiated seedlings is double. There are similarities and differences between the evolution of in vitro lavender explants, the bud having superiour values of caulogenesis and even propagation. The results show the effect of glycine aminoacid in the process of in vitro regenerating of lavander plants and also in delaying or even stopping of the auxine’s reaction ( for inducing rooting). We recomend testing glycine in medium for other species (having economic, ornamental values or spontaneous species, etc), in order to establish if it has or hasn’t the capacity to stop or reduce rootedness induced by auxine. On all versions the rooting is blocked by a small collar of callus generated at the bottom of the seedling (similar differentiation). As far as in vitro lavender cultivation is concerned, AIB medium (V2) has the best effect in propagation and, organogenesis and caulogenesis.
Authors and Affiliations
Eliza Agud, Vasile Laslo, Maria Zăpârţan, Aurelia Oneţ, Cristian Oneţ
Keywords
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