REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR MYCOBACTERIUM TUBERCULOSIS IN SERPIGINOUS CHOROIDITIS- A STUDY OF 29 CASES
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2014, Vol 3, Issue 3
Abstract
Purpose: A study of real time Polymerase Chain Reaction for Mycobacterium tuberculosis (M. tuberculosis) DNA in 29 cases of active serpiginous choroiditis. Design: Case control study. Methods: DNA extraction from the aqueous humor was carried out using QIAMP DNA extraction kit. Real- time Polymerase Chain reaction (RTPCR) for MTB was carried out using Genosen’s Mtb complex quantitative Real time PCR kit. All patients were also subjected to complete blood count, venereal disease research laboratory test, chest radiograph, QuantiFERON TB Gold test on the blood and polymerase chain reaction on a sample of aqueous humor. Results: Aqueous aspirate showed copies of mycobacterium tuberculosis DNA in one out of twenty nine cases of serpiginous choroiditis. Direct smear and culture for mycobacteria was negative in all cases. Conclusion: RTPCR identifies MTB DNA in suspected latent tuberculosis in serpiginous choroiditis with high specificity. Serpiginous choroiditis and multifocal choroiditis due to tuberculosis may resemble each other clinically but have distinct clinical features which can be confirmed by real time polymerase chain reaction performed on the aqueous humor The association between serpiginous choroiditis and tuberculosis would be a chance association or if present a rare association.
Authors and Affiliations
Radha Annamalai| Associate Professor, Department of Ophthalmology, Sri Ramachandra University, Porur, Chennai,India, Corresponding author email: drradhaannamalai@yahoo.co.in, Jyotirmay Biswas| Director of Uvea & Ocular Pathology Department, Sankara Nethralaya, Chennai,India, S Sudharshan| Consultant- Department of Uvea, Sankara Nethralaya, Chennai,India, R Gayathri | Postdoctoral fellow, L & T Microbiology Research Centre, Vision Research Foundation, KNBIRVO Building 41, College Road, Chennai - 600 006, K Lily Therese| Senior Professor and HOD, L & T Microbiology Research Centre, Vision Research Foundation, KNBIRVO Building 41, College Road, Chennai - 600 006, Viswanathan S| Professor of Ophthalmology, Muthukumaran Medical College, Chennai, India, Namitha Bhuvaneswari| Director and Professor of Ophthalmology, Regional Institute of Ophthalmology, Chennai, India
Keywords
Related Articles
- EP ID EP11264
- DOI 10.5958/2319-5886.2014.00391.9
- Views 319
- Downloads 14
Patient-Centered Care; Physicians’ View of Obstacles against and Ideas for Implementation
To implement proper family medicine practice and to get the best of it, the concept of patient-centered care (PCC) has to be put into use. Studies have found that one of the most important advantages of PCC is the incre...
COMPARATIVE STUDY FOR EVALUATING T SPOT-TB IN ANALYSIS BETWEEN LOW AND HIGH RISK SUBJECTS - A PILOT STUDY
Latent tuberculosis infection (LTBI) is a non- communicable asymptomatic condition, which might develop into active tuberculosis. Health care workers in contact with active TB cases are at high risk. Impact of exposure...
A Study to Evaluate the Role of Intravenous Immunoglobulin (IVIG) as an Adjuvant in the Management of Neonatal Sepsis in Preterm Babies
Introduction: Newborn children conceived before 32 weeks of incubation are genuinely immune defcient with cord blood centralization of IgG being not as much as half contrasted with those found in infants conceived at fu...
Prediction of Marital Dissatisfaction Based on Personality Characteristics and Social Relations in Couples of Students and Non-Students
Te aim of this research was to study the prediction of marital dissatisfaction based on personality characteristics and social relations in couples of students and non-students. For this purpose, using Cluster sampling,...
COLD AGGLUTININ INDUCED HEMOLYTIC ANEMIA IN A PATIENT WITH PULMONARY TUBERCULOSIS
Autoimmune hemolytic anemias (AIHA) are an uncommon group of disorders characterized by red cell destruction due to autoantibodies. Though usually idiopathic, AIHA is commonly associated with lymphoproliferative disord...