Regeneration of Indian maize genotypes (Zea mays L.) from immature embryo culture through callus induction
Journal Title: Journal of Applied and Natural Science - Year 2015, Vol 7, Issue 1
Abstract
Callus induction and regeneration ability of five maize genotypes UMI 757, UMI 615, UMI 112, UMI 285 and CO 1 and one promising maize hybrids COH(M) 5 were investigated using 14 days old immature embryos as explants. Callus induction depends on genotype, explants (age and size of explants), medium, type of auxin and their concentration. Explants grown on Murashige and Skoog (MS) medium supplemented with 1.5 mg/l 2, 4 - D (2,4 – dichloro phenoxy acetic acid), 0.3 mg/l kinetin with 30 g/l maltose showed the highest percentage of embryogenic callus induction. Among the six genotypes tested, COH(M) 5 maize hybrids have highest percentage of embryogenic calli. The embryogenic calli incubated on MS medium supplemented with 1.5 mg/l BAP (Benzyl Amino Purine), 0.2 mg/lNAA (Naphthalene Acetic Acid) with 1.0 mg/l kinetin was found to give the highest organogenesis response and regeneration of plantlets.
Authors and Affiliations
M. Malini, C. R. Ananadakumar, S. Hari Ramakrishnan
Keywords
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- EP ID EP282571
- DOI 10.31018/jans.v7i1.576
- Views 60
- Downloads 0
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