Regeneration of the Experimental Defect of the Long Bone of the Skeleton after Implantation β-Tricalcium Phosphate
Journal Title: Journal of Krishna Institute of Medical Sciences University - Year 2016, Vol 5, Issue 3
Abstract
Background: One of the main characteristics of calcium phosphate osteoplastic materials is the dynamics of rate of their resorption and replacement by the bone tissue of the regenerate. However, these data in the compact bone tissue for some drugs on the basis of β-tricalcium phosphate and electron microscopic characteristics of the structures of the regenerate are not represented in the scientific literature. Aim and Objectives: This study was designed to evaluate the healing of the defect of compact bone tissue after implantation of osteoplastic material «Calc-i-oss®» (β-tricalcium phosphate, granules in the size of 1-1.6 mm) with the definition of the dynamics of its resorption and morphological characteristics of the bone tissue of regenerate. Material and Methods: In the middle third of the femoral shaft of 24 white Wistar rats eight months of age with the weight of 250±10 g we reproduced perforated defect to the medullary canal, which was filled with β-tricalcium phosphate. After surgical intervention fragments of bones were examined on the 60th and 120th day by light microscopy with morphometry and scanning electron microscopy. Results: The study has established the absence of an inflammatory reaction in the area of the defect, symptoms of necrobiosis and necrosis of osteocytes in adjacent to the site of implantation maternal bone, as well as the location on the surface and inside of the particles β-tricalcium phosphate of osteogenic cells and the formation of bone tissue of regenerate. Osteoplastic material throughout the observation period was subjected to resorption and substitution by bone tissue of the regenerate, the ratio of which on the 60th day of the experiment was 25.72±2.06% to 74.28±2.06% and on the 120th day 18.31±1.54% to 81.69±1.54%. Conclusions: It can be concluded that β-tricalcium phosphate in the area of the defect of diaphysis of the femur shows high biocompatibility, osteoconductive properties, the ability to resorption and good integration with bone tissue of the regenerate.
Authors and Affiliations
A. Korenkov
Keywords
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