Relationship Between Elevated FVIII Level and 3' UTR Variations of F8 Gene in Turkish Patients with Venous Thrombosis
Journal Title: Artuklu International Journal of Health Sciences - Year 2022, Vol 2, Issue 1
Abstract
Objective: Venous Thrombosis (VT) is an important medical disorder caused by genetic and environmental factors. This study investigated the sequence variants in the 3' untranslated region (UTR) of Factor 8 gene in 30 patients with high FVIII plasma levels and 30 healthy individuals with normal FVIII plasma levels. Materials and Methods: The plasma levels of FVIII protein were measured in blood samples using the Static Timing Analyze Kit. The 3' UTR region of F8 gene was amplified by PCR using 6 primers pairs. Single strand conformation analysis (SSCA) and DNA sequencing analysis were carried out for determination of the sequence variants. Results: While the FVIII protein level was 200 ± 64 IU / dl in patients, it was detected as 120 ± 32 IU / dl in controls. In the 3 'UTR of the F8 gene were observed different SSCA patterns of some individuals and nucleotide change rs1050705 of the F8 gene. Therefore, we did not find a significant relationship between sequence variants in 3' UTR of F8 gene and elevated FVIII levels. Conclusions: VT does not seem to be associated with the sequence variant in 3' UTR of the F8 gene but small-scale studies will draw attention to studies on the 3' UTR regions of the genes to elucidate complex disorders such as venous thrombosis.
Authors and Affiliations
Mustafa Ay
Keywords
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- EP ID EP708329
- DOI http://dx.doi.org/10.29228/aijhs.10
- Views 69
- Downloads 0
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Değerli okuyucularımız, Artuklu International Journal of Health Sciences dergimizin ilk sayısını çıkartmanın heyecanını ve mutluluğunu siz değerli okuyucularımızla paylaşıyoruz. Dergimiz Mardin Artuklu Üniversitesi...
Relationship Between Elevated FVIII Level and 3' UTR Variations of F8 Gene in Turkish Patients with Venous Thrombosis
Objective: Venous Thrombosis (VT) is an important medical disorder caused by genetic and environmental factors. This study investigated the sequence variants in the 3' untranslated region (UTR) of Factor 8 gene in 30 pat...
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