Relationship between puerarin and urothelial carcinoembryonic antigen 1 expression in apoptosis and autophagy of esophageal cancer cells
Journal Title: Chinese Journal of Clinical Research - Year 2025, Vol 38, Issue 1
Abstract
"<b>Objective</b> To explore the molecular mechanism of puerarin on apoptosis and autophagy in esophageal cancer cells. <b>Methods</b> Human esophageal cancer EC109 cells were treated with puerarin at 0, 200, 500, 800 μmol/L. The cell proliferation,migration and invasion ability were measured by CCK-8 method, the scratch assay and transwell test, respectively. The expression levels of apoptosis-related proteins (pro-apoptotic proteins Bax and Bad, anti-apoptotic protein Bcl-2) and autophagy-related proteins p62, Beclin-1 and ATG5 were detected by Western blot. The expression level of urothelial carcinoembryonic antigen 1 (UCA1) was detected by real-time fluorescence quantitative polymerase chain reaction. EC109 cell lines stably overexpressing UCA1 were constructed by lentiviral transfection and divided into stable overexpressing UCA1 cells (UCA1-OE group) and negative control cells(UCA1-OENC group). The two groups of cells were treated with the same 500 μmol/L puerarin, and the expression levels of cells apoptosis and autophagy protein were detected using cellular immunofluorescence assay. <b>Results</b> Puerarin treatment with 200, 500 and 800 μmol/L significantly inhibited the proliferation, migration and invasion activities of EC109 cells ( P <0.01), promoted the expression of Bax, Bad, p62, Beclin-1 and ATG5 proteins ( P <0.01) and inhibited the expression of Bcl-2 protein ( P <0.01), all of which were concentration-dependent. Compared with 0 μmol/L (1.833±0.061), puerarin treatments of 200, 500 and 800 μmol/ L significantly inhibited the expression of UCA1 protein (the relative expression levels of UCA1 were 1.490±0.056, 1.120±0.044 and 0.783±0.015, respectively,F =274.876,P <0.01), which was also concentration-dependent. After 500 μmol/L puerarinin treatment, UCA1-OE cells overexpressing UCA1 showed lower expression levels of Bax, Bad, p62, Beclin-1 and ATG5, as well as higher expression levels of Bcl-2 compared to UCA1-OENC cells ( P <0.05). <b>Conclusion</b> Puerarin induces apoptosis and autophagy in esophageal cancer cells by inhibiting the expression of UCA1."
Authors and Affiliations
LI Jing, WANG Jumei, TIAN Jingrong, BI Lihua, GAO Yuanyuan, SHANG Hong
Keywords
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