Relationship of the recurrence after pterygium surgery with the presence of HSV, EBV, CMV, HPV, and BRAFV600E mutation
Journal Title: Офтальмологический журнал - Year 2019, Vol 0, Issue 1
Abstract
Background: Previously, we have reported on associations of pterygium with the presence of herpes simplex virus (HSV), Epstein Barr virus (EBV), cytomegalovirus (CMV), human papillomavirus (HPV), and the BRAFV600E somatic point. The current study attempted to examine the relationship between the recurrence during 1 year after pterygium surgery and the presence of these factors. Purpose: To identify the relationship between (a) recurrence rates after various surgical techniques for pterygium and (b) the presence of herpes viruses (HSV, EBV, and CMV), HPV 6, 11, 16 and 18 and BRAFV600E mutation. Materials and Methods: Two hundred and three patients (232 eyes) with a history of pterygium surgery underwent examination. They were divided into five groups based on the method of surgical treatment: Group 1 (the McReynolds transplantation only) of 49 eyes; Group 2 (the McReynolds transplantation, with adjuvant MMC 0.02% applied to the cornea for 30 sec) of 41 eyes; Group 3 (the technique of Arlt) of 49 eyes; Group 4 (the technique of Arlt, with adjuvant MMC 0.02% applied to the cornea for 30 sec) of 46 eyes; Group 5 of 47 eyes that were treated with our technique of pterygium excision involving the growth plate, with conjunctival autoplasty and adjuvant MMC 0.02%. Real-time PCR with PCR kits from DNK Tekhnologiya (Russia) were used to identify HSV, EBV, CMV, and HPV. PCR amplification was performed in a DT-lite real-time PCR system (DNK Tekhnologiya). Mutation detection was performed using TaqMan Mutation Detection Assays (Thermo Fisher Scientific) in a Real-Time PCR System 7500 (Applied Biosystems, USA). Results: Changing from traditional surgical methods (the McReynolds or Arlt method, alone or with adjunctive mitomycin C) to our methodology for pterygium decreased the recurrence rate at 1 year after pterygium surgery by 17.0% (or 2.1-fold), from 31.9% to 14.9% (χ2 = 5.32; р = 0.021). There was a common trend regarding the relationship between the presence of BRAFV600 mutation and pterygium recurrence in the groups: in the groups with traditional surgical methods, the recurrence rate in BRAFV600 mutation-positive patients varied from 73.3% to 81.2%, whereas the recurrence rate in BRAFV600 mutation-negative patients varied from 4.2% to 10.7% (p < 0.001). The mutation increased the risk of a recurrence within a year after pterygium surgery approximately 35.7-fold (OR=35.72; 95% CI, 15.71-81.24). Changing from traditional surgical methods to our methodology for pterygium decreased the recurrence rate at 1 year after pterygium surgery in BRAFV600 mutation-positive patients by 39.8% (or 2.1-fold), from 77.3% to 37.5% (χ2 = 116.92; р < 0.01). Statistical analysis found no relationship between the distribution of recurrences among the groups, and presence of either individual viruses or their combinations (p > 0.1 for all cases). Conclusion: The presence of HSV, EBV, CMV, HPV, and/or BRAFV600 mutation in pterygium tissue is a risk factor for recurrence after surgical treatment.
Authors and Affiliations
S. Rykov, K. Usenko, L. Denisiuk
Keywords
Related Articles
- EP ID EP490098
- DOI 10.31288/oftalmolzh2019138
- Views 82
- Downloads 0
Examination of school children’s threshold exposure duration for recognition of test objects (TEDRTO) for assessment of their visual fatigue
Background: Due to increased visual load during schooling, it is important to use a methodology for assessment of visual fatigue in children which would be understandable to them and which would not require much time. P...
Efficacy assessment of treatment of patients with stage III primary open-angle glaucoma using a combined method (phosphene electrical stimulation and photomyostimulation)
The purpose: to assess the efficacy of a combined method, including phosphene electric stimulation (FES) and photomyostimulation, in treatment of patients with stage III primary open-angle glaucoma (POAG) with compensate...
Structure of the chorioretinal complex in the rabbit eye after vitrectomy. Report 1. Vitreous cavity irrigation with different temperature solutions for 30 minutes
Background: It remains poorly understood what should be the temperature of the irrigating solution for intraocular surgery and how long it is reasonable to use irrigating solutions during vitrectomy. Purpose: To invest...
Patterns of development of retinal vascular pathology at remote time period after radiation exposure
Background. Retinal vascular system is extremely sensitive to ionizing radiation exposure and requires priority medical examination. Probability of new accident environmental contamination with radioactive material is a...
Ultrastructural changes in the rabbit chorioretinal complex following 577-nm laser photocoagulation
Background: There is no unanimously adopted approach to titrating laser parameters in subthreshold retinal laser photocoagulation without ophthalmoscopically visible fundus changes. Purpose: To investigate the ultrastru...