Relative Assessment of Some Toxins on Molecular Changes in the Protein Profile of Mugil Cephalus, Peaneaus Indicus and Protues PelagicusProcured from some Estuaries of South East Coast of Indian Ocean
Journal Title: International Journal of Oceanography & Aquaculture - Year 2018, Vol 2, Issue 6
Abstract
Marine organisms namely Mullet (Mugil cephalus), Crab (Portunus pelagicus), and Shrimp (Penaeus indicus) were collected during post-monsoon, pre-monsoon and monsoon from Vellar and Uppanar estuaries, South East Coast of Indian Ocean. Muscle of considered species was examined for a presence of toxic metals(Al, Cd, Cu, Pb and Zn), utilizing Inductively Coupled Optical Emission Spectrometry (ICP-OES). The outcome demonstrated the Aluminum concentration (12.176µg/g) had a most extreme level in considered marine organisms at Uppanar estuary took after by zinc (1.332 µg/g), copper (0.204 µg/g), lead (0.054 µg/g) and cadmium (0.004µg/g) when contrasted and Vellar estuary Al (2.084µg/g), Zn (0.307µg/g), Cu (0.145 µg/g), Pb (0.054µg/g) and Cd (0.026µg/g). None of the toxic metals examined at Vellar and Uppanar estuaries was exceeding acceptable levels. The impact of these trace metals on protein were investigated quantitatively and subjectively. Likewise, utilizing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and total protein estimation. The most level of total protein was found in mullet muscle of (381.8mg/ml) in monsoon and shrimp (377.0mg/ml) in pre-monsoon at Vellar estuary and the base level was found in mullet (211.8mg/ml) at Uppanar estuary in the same season. Total protein level was watched lower in all test species of Uppanar estuary than the species of Vellar estuary. SDS-PAGE examination as protein profile demonstrating the little varieties in protein divisions in correlation among species gathered from two localities.
Authors and Affiliations
Hassan Sulieman MA1, El Amin S2
Keywords
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- EP ID EP562217
- DOI 10.23880/ijoac-16000155
- Views 85
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