Repair of Articular Cartilage Defect Using NaOH-treated PLGA-[ASP-PEG]n Scaffolds Loaded Adipose-derived Stem Cells and Bone Morphogenetic Protein-2
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2014, Vol 6, Issue 2
Abstract
The aim of this study was to evaluate the feasibility of NaOH-treated PLGA-[ASP-PEG]n scaffolds with bone morphogenetic protein 2 (BMP-2) modified adipose derived mesenchymal stem cells (ADSCs) in repairing articular cartilage defects. To improve functions of chondrocytes including their adhesion, growth, differentiation and extracellular matrix synthesis, poly(lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol [PLGA-[ASP-PEG]n] scaffolds were modified via chemical etching techniques using 1 N NaOH for 10 min. Articular cartilage defects of 3-mm diameter and 5-mm depth were created in the patellar groove of the femoral articular cartilage of rabbit’s knee joints. The injury sites were treated with BMP-2 modified ADSCs/PLGA-[ASP-PEG]n and BMP-2 modified ADSCs/NaOH-treated PLGA-[ASP-PEG]n, or left empty. The defect area was evaluated using gross and histological examination. Results demonstrated that NaOHtreated PLGA-[ASP-PEG]n scaffolds enhanced chondrocyte functions and present faster and more effective chondrogenesis compared to non-treated scaffolds. The present study demonstrated that NaOH-treated PLGA-[ASP-PEG]n scaffolds loaded BMP-2 modified ADSCs might be a promising treatment in repair of articular cartilage defect.
Authors and Affiliations
Bi Zhang
Keywords
Related Articles
- EP ID EP183968
- DOI -
- Views 95
- Downloads 0
The Revolution in Endodontic Imaging: Extraoral Periapical Radiography
Aim To evaluate the accuracy of extraoral periapical radiography in all phases of endodontic therapy and to determine the magnification error by comparing it with the radiographs obtained from the standard intraoral appr...
Reason Analysis for Suspension/Early Termination of Drug Clinical Trial
Object To collect a scientific basis for improving the quality and success rate of drug clinical trials by analyzing the reasons for the suspension or termination of clinical trials in China and abroad. Method Drug Clini...
Cytomorphological Evaluation of 1,000 Cases of Unhealthy Cervix to Assess Present Role of Pap Smear Screening in Developing Country
Introduction Last few decades saw a Pap smear becoming synonymous with early detection of cervical carcinoma, leading to its rapid decline worldwide. However, newer screening modalities like liquid cytology have been emb...
Evaluation of Habit Patterns and Clinical Findings of Oral Submucous Fibrosis in South Indian Population
Objective To evaluate the habit patterns and clinical features of oral submucous fibrosis. Materials and Methods A hospital-based cross sectional study was conducted in a sample of 75 subjects with clinically diagnosed o...
Detection of antibacterial activity of Querus infectoria against MRSA isolates
Abstract: Background:- Methicillin-resistant Staphylococcus aureus (MRSA) has been well-documented as a major cause of hospital infections. The aim of the present work was to evaluate the antibacterial potential of aqueo...