REPEATED ACUTE STRESS INDUCED ALTERATIONS IN CARBOHYDRATE METABOLISM IN RAT
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2010, Vol 6, Issue 3
Abstract
Acute stress induced alterations in the activity levels of rate limiting enzymes and concentration of intermediates of different pathways of carbohydrate metabolism have been studied. Adult male Wistar rats were restrained (RS) for 1 h and after an interval of 4 h they were subjected to forced swimming (FS) exercise and appropriate controls were maintained. Five rats were killed before the commencement of the experiment (initial controls), 5 control and equal number of stressed rats were killed 2 h after RS and remaining 5 rats in each group were killed 4 h after FS. There was a significant increase in the adrenal 3β- hydroxy steroid dehydrogenase activity following RS, which showed further increase after FS compared to controls and thereby indicated stress response of rats. There was a significant increase in the blood glucose levels following RS which showed further increase and reached hyperglycemic condition after FS. The hyperglycemic condition due to stress was accompanied by significant increases in the activities of glutamate- pyruvate transaminase, glutamate- oxaloacetate transaminase, glucose -6- phosphatase and lactate dehydrogenase and significant decrease in the glucose -6- phosphate dehydrogenase and pyruvate dehydrogenase activities, whereas pyruvate kinase activity did not show any alteration compared to controls. Further, the glycogen and total protein contents of the liver were decreased whereas those of pyruvate and lactate showed significant increase compared to controls after RS as well as FS. The results put together indicate that acute stress induced hyperglycemia results due to increased gluconeogenesis and glycogenolysis without alteration in glycolysis. The study first time reveals that after first acute stress exposure, the subsequent stressful experience augments metabolic stress response leading to hyperglycemia. The results have relevance to human health as human beings are exposed to several stressors in a day and such an experience might lead to insulin resistance because prolonged hyperglycemic condition is known to cause insulin resistance.
Authors and Affiliations
R Nirupama, M Devaki, H Yajurvedi
Keywords
Related Articles
- EP ID EP139555
- DOI -
- Views 126
- Downloads 0
Evaluation of the Effect of Oxidative Stress on Roots of Red Beet (Beta vulgaris L.)
In the present investigation it was studied the effect of oxidative stress on root of red beet. The degree of stress exposure was assessed at various levels: in tissues of roots (conductometric method), on isolated vacuo...
Euhalophyte Eryngium maritimum L.: the Microstructure and Functional Characteristics
The microstructure of the leaf surface, lipids composition, pigments spectrum, biological and photosynthetic activity, and hormones status of euhalophyte [i]Eryngium maritimum[/i] L., one of the most salt-tolerant plants...
Low Temperature Stress Induced Changes in Biochemical Parameters, Protein Banding Pattern and Expression of Zat12 and Myb Genes in Rice Seedling
Low temperature stress is one of the main abiotic factors that reduce the productivity of many crops in hilly areas around the world. In this study, rice seedling were exposed to low temperature stress (control, 0°C, -2°...
Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L.) under salinity stress
Application of salicylic acid (SA) as a phytohormone has been increased due to resistance to stresses such as salt stress. Pot experiments were conducted to determine the effect of exogenous salicylic acid application on...
Interactive effects of rice residue and water stress on growth and metabolism of wheat seedlings
In the present study effects of rice residue with and without water stress were studied on [i]Triticum aestivum[/i] L. cv. Shatabadi. The mixture of residue and garden soil in 1:1 ratio was considered as 50% (R[sub]1[/su...