Resazurin Microplate Assay: Rapid Assay for Detection of Methicillin Resistant Staphylococcus aureus
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 4
Abstract
The increasing methicillin resistant Staphylococcus aureus (MRSA) infections pose a serious threat. Accurate and rapid detection of methicillin resistance is important for ensuring the prompt start of antibiotherapy and control of MRSA in the hospitals. Molecular detection of mecA gene is the gold standard for identification of MRSA isolates. However, many laboratories don’t have the capacity for molecular techniques. Resazurin is a dye used as an oxidation reduction indicator in bacterial cell viability assays. The aim of the study was to introduce resazurin microplate assay (REMA) as a new colorimetric method for identification of MRSA. The study included 100 Staph. aureus clinical isolates which were tested for their susceptibility to methicillin by the cefoxitin (30ug) disc diffusion (DD) method and REMA. Detection of the mecA gene was done by PCR. Out of the 100 studied isolates, 65% were MRSA by DD and the REMA. A highly significant association was found between the results of DD method and the results of REMA. The mecA gene was detected in 64/65 of the MRSA isolates. There was a highly significant association between the results of REMA and that of the PCR for the mecA gene. It is concluded that the REMA is a sensitive and specific assay for rapid phenotypic detection of MRSA in poor resource laboratories.
Authors and Affiliations
Hala Mahmoud Hafez, Dalia H Abd El Hamid, Dina Tarek, Fatma Al-Zahraa M. Gomaa
Keywords
Related Articles
- EP ID EP569963
- DOI 10.20546/ijcmas.2017.604.020
- Views 77
- Downloads 0
Gene Action and Combining Ability in Rice (Oryza sativa L.) Involving Indica and Tropical Japonica Genotypes
Combining ability for yield and its components was analyzed in a 8×8 diallel crosses (excluding reciprocals), involving 3 indica and 5 tropical japonica rice. The GCA and SCA were significant for all the characters, indi...
Isolation and Identification of Extra-aquatic, Pathogenic Fungi from the Polluted Water of Munneru River, Telangana, India
Munneru is a small River and flows through Dornakal Eru, Kaamanchkal and Daanavaayigudem areas of Khammam district, Telangana, India. Munneru is a major water source, the river is so much polluted with the domestic, fact...
Effect of Moisture Stress on Key Physiological Parameters in Sunflower Genotypes
A study was conducted to investigate the effect of drought on physiological characteristics in twelve sunflower genotypes. Moisture stress treatment were imposed at flower bud initiation stage (irrigation withheld from 4...
Problems Faced by NGOs Trainees in Adoption of Entrepreneurial Activities
The objective of the present study was to identify the problems faced by NGOs trainees in adoption of entrepreneurial activities. The study was conducted in Gogunda and Kherwara panchayat samities of Udaipur district of...
Studying Efficiency Inhibition of Some Medicinal Plant Extracts against Some Fungal
The present study impression concentrations different from extract alcohol some of the medicinal plants mint (Mentha spicata L.) basil (Ocimum basilicum L.) Dill (Anethum graveolens) agent isolation the fungal and which...