Resazurin Microplate Assay: Rapid Assay for Detection of Methicillin Resistant Staphylococcus aureus
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 4
Abstract
The increasing methicillin resistant Staphylococcus aureus (MRSA) infections pose a serious threat. Accurate and rapid detection of methicillin resistance is important for ensuring the prompt start of antibiotherapy and control of MRSA in the hospitals. Molecular detection of mecA gene is the gold standard for identification of MRSA isolates. However, many laboratories don’t have the capacity for molecular techniques. Resazurin is a dye used as an oxidation reduction indicator in bacterial cell viability assays. The aim of the study was to introduce resazurin microplate assay (REMA) as a new colorimetric method for identification of MRSA. The study included 100 Staph. aureus clinical isolates which were tested for their susceptibility to methicillin by the cefoxitin (30ug) disc diffusion (DD) method and REMA. Detection of the mecA gene was done by PCR. Out of the 100 studied isolates, 65% were MRSA by DD and the REMA. A highly significant association was found between the results of DD method and the results of REMA. The mecA gene was detected in 64/65 of the MRSA isolates. There was a highly significant association between the results of REMA and that of the PCR for the mecA gene. It is concluded that the REMA is a sensitive and specific assay for rapid phenotypic detection of MRSA in poor resource laboratories.
Authors and Affiliations
Hala Mahmoud Hafez, Dalia H Abd El Hamid, Dina Tarek, Fatma Al-Zahraa M. Gomaa
Keywords
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- EP ID EP569963
- DOI 10.20546/ijcmas.2017.604.020
- Views 71
- Downloads 0
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