Research on dehydrogenation of gomphrenin in heated ammonia solutions of organic solvents
Journal Title: Challenges of Modern Technology - Year 2017, Vol 8, Issue 1
Abstract
In this report, the influence of ammonia on dehydrogenation of gomphrenin pigment, isolated from purple inflorescences of Gomphrena globosa L., in selected organic solvents (methanol, ethanol, acetone and acetonitrile) at 40 0C is presented. Betacyanins are water-soluble, vacuolar plant pigments. Due to their numerous pro-health properties, they can be applied in the pharmaceutical industry and as food colorants. However, as most of natural products, they are less stable than synthetic dyes, therefore, further studies need to be carried out on their stability, but also on determination of their degradation products as well as degradation mechanisms. For the experiments of the ammonia treatment, prolonged isolation of gomphrenin from the plant material including extraction, preliminary purification, preparative HPLC fractionation, concentration and liofilization were performed. The tested pigment dissolved in organic solvents was treated by ammonia and the samples for analysis were taken during and after reaction. For identification of obtained derivatives of gomphrenin, high-performance liquid chromatography with diode-array and mass spectrometric detection (LC-DAD-ESI-MS) was performed. As a result of heat processing, the mixture of decarboxylated and dehydrogenated gomphrenin derivatives was detected. Based on the experimental results, it can be stated that gomphrenin is characterized by the greatest stability in methanolic solutions while it is the most labile in the acetonitrilic ones.<br/><br/>
Authors and Affiliations
Natalia Szmyr, Sławomir Wybraniec
Keywords
Related Articles
- EP ID EP346612
- DOI -
- Views 68
- Downloads 0
Effect of the strong magnetic field on copper adsorption processes on activated carbon
In the presented studies an influence of the strong static magnetic field on the process of copper adsorption on activated carbon was analysed. The magnetic field was generated by the annular neodymium magnets, placed at...
Towards safe campus environments through environmental design: two universities as case studies
Universities are often considered to be safe sanctuaries. However, many higher education institutions have increasingly been confronted with crime and unrest. Violence and other crimes on campuses are currently an intern...
Research on stability of gomphrenin pigments influenced by Cu2+ ions
This paper concerns the results of dehydrogenation of gomphrenin pigments isolated from the purple flowers of Gomphrena globosa L. depending on pH and Cu2+ activity. Betalains are the plant dyes divided into red-violet b...
Application of finite element method for the analysis of a prefabricated post-and-core – tooth system
In recent years in the area of prosthetic treatment, great emphasise was put on leaving a residual dentition inside mouth. In a teeth after endodontic treatment or with broken crown a healthy root is a natural implant th...
The microstructure and properties of the 7CrMoVTiB10-10 steel welded joint
7CrMoVTiB10-10 is modern steel used to build boilers across Europe. Welding is very important operation during the process of building boilers. Microstructure and properties in the heat affected zone are changed by weldi...