RESEARCH ON THE CONTENT OF STAPHYLOCOCCUS SPP. MILK FROM PATIENTS WITH SUBCLINICAL MASTITUDE OF COWS
Journal Title: Наукові доповіді НУБіП України - Year 2017, Vol 6, Issue 6
Abstract
One of the most dangerous diseases for dairy herds is staphylococcal mastitis. With the secretion of the mammary gland, the pathogen spreads in the environment, and is long enough in the air, water, soil, on various items of consumption, which can be the factors of transmission of the pathogen to susceptible individuals. Studies on the staphylococcal content of milk, selected from healthy and patients with subclinical mastitis of cows. Materials and methods. We examined 160 samples of milk from healthy and patients with subclinical mastitis of cows from 2 farms of Kyiv and farms in Khmelnytskyi oblasts. The research of samples and the allocation of pure culture was carried out by the Drigalsky method on a dense nutrient medium of the Beard-Parker agar. A total of 160 samples were investigated; Staphylococcus cultures were isolated in 59 samples, which is 36.9%. Including from 14 samples of milk from cows suffering from subclinical mastitis from the Khmelnytsky region microorganisms of the genus Staphylococcus spp. was detected in 2 cases, which is 14.29%. In 16 samples of milk of patients with subclinical mastitis of cows from the economy of the Kiev region, staphylococci were also detected in 2 cases, which is 12.5%. In another farm of the Kiev region of selected 130 samples staphylococci were found in 41 cases, which is - 31.5%. Of these, from clinically healthy animals, staphylococci were isolated in 31.7% of cases and from patients with subclinical mastitis in 68.3%. Conclusions of the 160 samples of milk from clinically healthy and patients with subclinical mastitis of cows, 59 cultures of microorganisms were identified that were identified as representatives of the genus Staphylococcus. The directions of our further research will be: the next type of their identification, the study of the biological properties of staphylococci - the reaction of plasma coagulation, fermentation of mannitol; determination of antibiotic resistance of isolated strains, ability to biofilm formation.
Authors and Affiliations
Y. Y. Vishovan
Keywords
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