RESPONSES OF BRAIN TISSUES AGAINST HYPOXIC CONDITION IN HEMORRHAGIC STROKE PATIENTS: NEUROGLOBIN EXPRESSION IN BRAIN TISSUE AND PLASMA
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 2
Abstract
Objective: Strokes remain a significant health concern and are the highest cause of mortality and physical or mental disability in productive and the elderly hospitalized patients in Indonesia. Neuroglobin (Ngb) mostly located in the central and peripheral nervous system, predicted enhanced neuronal survival under hypoxic condition, such as in a stroke. The aim of this study is to observe the response of the brain tissue of hemorrhagic stroke patients against hypoxic/ischemic conditions. The objectives are to recognize the pattern of Ngb expression in the brain tissue and plasma of hemorrhagic stroke patients, and furthermore, to compare the level of Ngb in the brain tissue and plasma of hemorrhagic stroke patients.Methods: This is an observational study with consecutive sampling methods using cerebral cortex and the blood of hemorrhagic stroke patients, who underwent craniotomies to evacuate hematomas at Cipto Mangunkusumo Hospital (RSCM) and other hospitals in Jakarta. Ngb expression was measured in brain tissue and blood using real time reverse transcription polymerase chain reaction, while the ELISA method was adopted to measure Ngb protein in plasma.Results: Hypoxia/ischemia in the brain tissue of hemorrhagic stroke patients increased the expression of Ngb in brain tissue compared to the blood. The level of Ngb protein in plasma of hemorrhagic stroke patients increased significantly compared to normal subjects; however, there is no significant difference between the plasma and brain tissue of hemorrhagic stroke patients.Conclusion: Hypoxia/ischemia in hemorrhagic stroke patients increases the expression of Ngb mRNA and protein level.Keywords: Neuroglobin, Hypoxia, Hemorrhagic stroke.
Authors and Affiliations
Ninik Mudjihartini
Keywords
Related Articles
- EP ID EP605480
- DOI 10.22159/ajpcr.2017.v10i2.15971
- Views 96
- Downloads 0
COLOR INTENSITY AND ANTIOXIDATIVE CAPACITY OF FOUR NATURAL MONOFLORAL HONEY FROM MALDA, INDIA
Objective: The objective of the study was to explore the color intensity and antioxidative capacity of mango honey available from local niches, in and around Malda, West Bengal, India. Methods: Four mango honey samples:...
SYNTHESIS OF COPPER OXIDE NANOPARTICLES BY CHEMICAL PRECIPITATION METHOD FOR THE DETERMINATION OF ANTIBACTERIAL EFFICACY AGAINST STREPTOCOCCUS SP. AND STAPHYLOCOCCUS SP.
Objective: To determine antimicrobial efficacy of copper oxide nanoparticles (CuO NPs) against Streptococcus sp. and Staphylococcus sp. Methods: CuO NPs were synthesized using chemical precipitation method. The reducing...
EVALUATION OF IN VIVO IMMUNOMODULATORY ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF EULOPHIA NUDA L.
Objective: The aim of this study was to evaluate the in vivo immunomodulatory activity of an aqueous and ethanolic extract of dried tubers of Eulophia nuda (ENA and ENE).Methods: Effect of both the extracts was evaluated...
THE EFFECT OF MAHKOTA DEWA (PHALERIA MACROCARPA) (SCHEFF.) FRUIT PERICARP EXTRACT ON INOS IN MICE COLON INTERMITTENTLY-INDUCED BY DEXTRAN SODIUM SULFATE
  Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced b...
RISK FACTORS FOR NON-COMMUNICABLE DISEASES AMONG RURAL ADOLESCENTS: A SCHOOL-BASED CROSS-SECTIONAL STUDY
 Objective: Non-communicable diseases (NCD) in recent times have attained a magnitude of epidemic proportion, with increasing number ofadolescence being reported as obese. The risk factors for NCD such as obesity, seden...