Restoration of immune system function is accelerated in immunocompromised mice by the B-cell-tropic isoxazole R-11.
Journal Title: Pharmacological Reports - Year 2012, Vol 64, Issue 2
Abstract
Background: Restoration of impaired immune response in immunocompromised patients is a crucial problem. In this study we evaluated the efficacy of isoxazole R-11 in reconstitution of the immune response in immunosuppressed mice. Methods: Mice were given a sublethal dose (250 mg/kg b.w.) of cyclophosphamide (CP). The cellular immune response to ovalbumin (OVA) and the humoral immune response to sheep erythrocytes (SRBC) were generated. R-11 was administered at repetitive, intraperitoneal doses (20 μg/mouse) until determination of the immune responses: 7 and 15 doses on alternate days for cellular and humoral immune response, respectively. For phenotypic studies R-11 was given per os, at a single dose of 20 μg/mouse. The ability of R-11 to affect interleukin- 6 (IL-6) production was determined in the whole human blood cell culture. Results: R-11 increased the content of CD19+ cells in the spleens and lymph nodes with a concomitant decrease of CD3+ and CD4+ cells. The compound significantly accelerated restoration of both cellular and humoral immune responses, elevated the numbers of circulating leukocytes and splenocytes and normalized the blood cell picture. Supplementary experiments showed that R-11 was not toxic with regard to human peripheral blood mononuclear cells (PBMC) and that it upregulated IL-6 production in blood cell culture stimulated with lipopolysaccharide (LPS). Conclusions: We demonstrated that R-11 is likely a B-cell tropic agent which can restore both cellular and humoral immune responses in immunocompromised mice and may have a potential to be applied in therapy of immunocompromised patients.
Authors and Affiliations
Michał Zimecki, Jolanta Artym, Maja Kocięba, Bożena Obmińska-Mrukowicz, Marcin Mączyński, Stanisław Ryng
Keywords
Related Articles
- EP ID EP161131
- DOI -
- Views 65
- Downloads 0
Hemostatic effects of simvastatin in subjects with impaired fasting glucose.
The aim of our study was to compare the effect of simvastatin on a range of hemostatic variables in subjects with impaired fasting glucose (IFG) and isolated hypercholesterolemia. We enrolled 28 subjects with IFG, 25 pri...
Effects of subcutaneous and intracerebroventricular injection of physostigmine on the acute corneal nociception in rats.
The present study investigated the effects of subcutaneous (sc) and intracerebroventricular (icv) injections of physostigmine (a cholinesterase inhibitor), atropine (an antagonist of muscarinic cholinergic receptors) and...
Search for drugs of the combined anti-inflammatory and anti-bacterial properties: 1-methyl-N'-(hydroxymethyl)nicotinamide.
It has already been reported that 1-methylnicotinamide (MNA(+)), a primary metabolite of nicotinamide (vitamin B(3)), possesses remarkable anti-inflammatory properties [3]. This communication shows that 1-methyl-N'-(hydr...
LC/MS/MS evaluation of cocaine and its metabolites in different brain areas, peripheral organs and plasma in cocaine self-administering rats.
We employed a cocaine intravenous self-administration model based on positive reinforcement of animals' instrumental reactions (i.e., lever pressing) rewarded by a dose of the drug. We also carried out simultaneous chara...
Responsiveness of 5-HT(2C) receptors in repeatedly diazepam-injected rats: a behavioral and neurochemical study.
The role of 5-hydroxytryptamine (serotonin; 5-HT)(2C) receptors in anxiety and the anxiolytic effects of drugs is well documented. In view of the withdrawal anxiety associated with repeated diazepam intake, the present s...