Restriction Fragment Length Polymorphism for the Stat5a and Fgf2 Genes in Three Sudanese Zebu Cattle.
Journal Title: Global Journal of Animal Scientific Research - Year 2015, Vol 3, Issue 3
Abstract
Two genes named: Signal Transducer and Activator of Transcription 5 A (STAT5A) and fibroblast growth factor 2 (FGF2) were typed in 90 animals representing three Sudanese cattle Zebu breeds (Fuga, Kenana and Butana). Blood samples were collected on vacutainer tubes supported with EDTA to prevent blood clotting, DNA were isolated and purified using standard phenolchlorophorm method. Polymerase chain reactions were performed using specific primers for each gene under study. Each gene PCR products were subject to cut with specific restriction enzyme: BstEII for STAT5A and Csp6I for FGF2 gene. The results showed that amplification of the STAT5A gene produced a PCR product of 820 bp length, cut with restriction enzyme revealed the presence of two alleles: C (0.04) and G (0. 96) which produced two genotypes: CG (0.08) and GG (0.92) while the CC genotype was absent in all the breeds under study. The amplification of FGF2 gene produced a PCR product of 207 bp length, cut with restriction enzyme revealed the presence of two alleles: A (0.973) and G (0.973) which produced two homozygous genotypes: AA (0.887) and AG (0.114) while the GG genotype was absent in all the breeds under study. The study proved the possibility of using RFLP technique in genetic analysis and some quantitative trait loci studies if productive data is available. The results also showed a significant amount of genetic variation is maintained in Sudanese Zebu cattle breeds.
Authors and Affiliations
Ibrahim Hummeda, Sally S. Alam, Abdel Aziz A. Makkawi, Salah-Eldein A. Sid-Ahmed, Ahmed S. Abdoon, Omaima M. Kandil, Mohamed Saber Hassanane
Keywords
Related Articles
- EP ID EP29151
- DOI -
- Views 304
- Downloads 5
A Study on the Specialization of Anemone Fishes Using Molecular Genetics Techniques.
This research investigated the specialization of anemone fishes and the phylogenetic relationships among 12 species of the genus Amphiprion and between two species of the genus Premnas using the mtDNA D-LOOP sequence....
Effects of Supplementation with Ficus Sycomorus (Shola) on Performances of Washera Sheep Fed Natural Pasture Hay
The experiment was conducted to evaluate the effect of supplementations with F. sycomorus leaf, fruit and their mixtures on digestibility of sheep fed basal diet hay, The experiment was carried out at Gish Abay in Seke...
Effects of Albizia Julibrissin Leaf Meal-Based Diet on Carcass and Sensory Characteristics of Broiler Chickens.
The carcass and sensory characteristics of broiler chicken fed Albizia julibrissin leaf meal (AJLM) was studied. A total of 24 (6 from each treatment) 8 weeks old broiler chickens were randomly selected from 120 broil...
Canonical Correlation Analysis Relating Age At First Egg, Bodyweight At First Egg And Weight Of First Egg With Egg Production At Different Periods In A Strain Of Layer Type Chicken
The relationship between age at first egg (AFE), bodyweight at first egg (BWFE), weight of first egg (WFE), with egg numbers recorded at 20–28 weeks (EN1), 28 – 35 weeks (EN2) and 35 – 42 weeks (EN3) was evaluated usi...
Ginger (Zingiber Officinale) Root Powder as Natural Feed Additive for Broiler Chicks
The study targeted effect of addition of ginger root powder as natural feed additive on growth performance and blood constituents of broiler chicks. One hundred and sixty unsexed one day-old broiler chicks strain (Ross...