Rickettsia rickettsii isolation from naturally infected Amblyomma parvum ticks by centrifugation in a 24-well culture plate technique
Journal Title: Open Veterinary Journal - Year 2013, Vol 3, Issue 2
Abstract
Rocky Mountain spotted fever is an acute illness caused by Rickettsia rickettsii (R. rickettsii) and is transmitted by the bite of ticks of the genera Dermacentor, Amblyomma and Rhipicephalus. The illness results in a high mortality rate and may be easily confused with other febrile syndromes. In Yucatan State, Mexico, childhood cases with a high mortality have been reported. In this work we report the isolation of a Mexican R. rickettsii strain from a tick egg mass using an alternative method for Rickettsia isolation with 24-well plates. We also identified a potential vector of R. rickettsii in the southeast of Mexico, which is Amblyomma parvum.
Authors and Affiliations
K. Dzul-Rosado| Regional Research Center “Dr. Hideyo Noguchi”, Autonomous University of Yucatan, Mexico, G. Peniche-Lara| Faculty of Medicine, Interinstitutional Unit of Clinical and Epidemiological Research. Autonomous University of Yucatan, Mexico, R. Tello-Martín| Regional Research Center “Dr. Hideyo Noguchi”, Autonomous University of Yucatan, Mexico, J. Zavala-Velázquez| Faculty of Medicine, Interinstitutional Unit of Clinical and Epidemiological Research. Autonomous University of Yucatan, Mexico, R. de Campos Pacheco| Federal University of Mato Grosso, Laboratory of Veterinary Parasitology, Department of Basic Science and animal production, Faculty of Agronomy, Animal Medicine and zootechny, Brazil, M. B. Labruna| Parasitic Disease laboratory, Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechny, São Paulo University, São Paulo, SP, Brazil, E. C. Sánchez| Government Health Services, Hospital Agustín O´Horan SSY, Calle Itzaes s/n. Col Centro, Merida Yucatan, Mexico, J. Zavala-Castro| Regional Research Center “Dr. Hideyo Noguchi”, Autonomous University of Yucatan, Mexico
Keywords
Related Articles
- EP ID EP9289
- DOI -
- Views 415
- Downloads 26
Developmental ossification sequences of the appendicular and axial skeleton in Kuttanad duck embryos (Anas platyrhynchos domesticus)
The processes of ossification sequences are poorly investigated for birds in general, even for domestic and experimental species and when it comes to the waterfowl it is almost negligible. Such sequences constitute a ri...
Expression of various sarcomeric tropomyosin isoforms in equine striated muscles
In order to better understand the training and athletic activity of horses, we must have complete understanding of the isoform diversity of various myofibrillar protein genes like tropomyosin. Tropomyosin (TPM), a coile...
Role of bovine herpesvirus type 5 (BoHV-5) in diseases of cattle. Recent findings on BoHV-5 association with genital disease
Bovine herpesvirus type 5 (BoHV-5) belongs to the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. This virus is a major causative agent of non-suppurative meningoencephalitis in young cattle. I...
Cystolithiasis in a Syrian hamster: a different outcome
A 14-month-old intact male Syrian hamster was admitted for lethargy and hematuria. A total body radiographic image and abdominal ultrasonography showed the presence of a vesical calculus. During cystotomy, a sterile uri...
Laboratory reference intervals for systolic blood pressure, rectal temperature, haematology, biochemistry and venous blood gas and electrolytes in healthy pet rabbits
Prospective data from 86 healthy pet rabbits were evaluated to establish reference intervals for hematology, biochemistry, urinalysis, venous blood gas and electrolytes, rectal temperature and systolic blood pressure. R...