Role of follicle-stimulating hormone and estradiol benzoate in recovering spermatogenesis in tamoxifen-injured rats
Journal Title: Asian Pacific Journal of Reproduction - Year 2018, Vol 7, Issue 6
Abstract
Objective: To evaluate the effects of follicle-stimulating hormone (FSH) and estradiol benzoate (EB) on the recovery of spermatogenesis, histology, sexual hormones levels and testicular gene expression in testes of tamoxifen-injured rats. Methods: Forty adult rats were divided into eight groups in a factorial arrangement of tamoxifen and hormonal treatments. Half of the groups orally received 0.6 mg/kg tamoxifen, and 30 d later tamoxifen and no- tamoxifen groups (controls) were paired and assigned into four hormonal treatments with daily intramuscular injections for 10 consecutive days: 1 mL saline (control); 7.5 IU FSH; 12 µg/kg EB; and 7.5 IU FSH+12 µg/kg EB. One day after the last treatment, spermatozoa were recovered from epididymis, blood was processed for sex hormones concentration (testosterone, FSH and luteinizing hormone) and testes were processed for histology and RNA extraction for expression of genes related to apoptosis [caspase 3, inducible nitric oxide synthase (iNOS) and B-cell lymphoma-2 (Bcl-2)]. Results: Control groups did not show significant changes in most parameters, but hormonal treatments decreased caspase 3 and iNOS and increased Bcl-2 expression. Tamoxifen significantly decreased counts, motility and viability of spermatozoa, Bcl-2 expression and sex hormones. It increased intertubular space, caspase 3 and iNOS expression, and induced seminiferous tubular atrophy. The hormonal treatments reverted spermatogenesis, hormonal levels and histology compared with controls, however not attaining the same sperm quality as controls. Conclusions: Tamoxifen is clearly detrimental to spermatogenesis and overall testicular structure and function, whereas hormonal therapy with FSH and EB can improve testicular function and revert tamoxifen-induced azoospermia.
Authors and Affiliations
Ali Olfati, Gholamali Moghaddam, Kaniaw Rafat Khafar, Ali Rooz Mojtahedin, Azad Abdolahzadeh
Keywords
Related Articles
- EP ID EP417132
- DOI 10.4103/2305-0500.246342
- Views 205
- Downloads 0
Functions of follicular and marginal zone B cells in pregnancy
No
Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation
Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supp...
Cryopreservation of cattle semen using coconut water extender with different glycerol concentrations
Objective: To investigate the effect of coconut water with a lone concentration and different concentrations of glycerol on chilled and cryopreserved cattle semen characteristics. Methods: Semen was collected from five m...
Seasonal effect on physiological, reproductive and fertility profiles in breeding mithun bulls
Objective: To analyse the seasonal effect on physiological parameters, reproductive profiles and in vitro fertility in breeding mithun bulls. Methods: A total of ten adult mithun bulls age of 5 to 6 years old with good b...
Pregnancy outcome of lactating dairy cows assigned for Presynch-Ovsynch synchronization program and inseminated either at detected standing heat or at scheduled fixed time
Objective: To determine if insemination at standing heat results in a similar or higher pregnancy rate compared with fixed time artificial insemination, and to study some factors affecting the pregnancy rate. Methods:...