Role of SAP7 in Hypha Formation and Adhesion to Colorectal Carcinoma Caco-2 (ATCC)
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 3
Abstract
Secreted aspartic proteases (Saps) are considered as key virulence factors of [i]Candida albicans[/i]. Hopefully our outlook will widen the knowledge of [i]SAP7’[/i]s role in [i]C. albicans[/i] pathogenesis. The goal of our study was to investigate [i]SAP7[/i] expression during [i]C. albicans[/i] adhesion to intestinal human cells. Another objective was to study the role of [i]Sap8-10[/i] and transcriptional regulators: [i]EFG1[/i] and [i]CPH1,[/i] using the mutants: [i]Δsap[/i], [i]Δefg1[/i], [i]Δcph1 [/i]during growth on the Caco-2 monolayer. [i]SAP7[/i] expression was analyzed using RT-PCR; relative quantification was normalized against [i]ACT1[/i] in cells after growth on Caco-2. Adherence assay of [i]C. albicans[/i] to Caco-2 was performed in 24-well-plate. Results proved that [i]SAP7[/i] can play a role during the initial adaptation of [i]C. albicans[/i] to intestinal tract and it decreases over time. Up-regulation of [i]SAP7[/i] occured in the absence of [i]SAP8[/i] and [i]SAP10[/i] (genetic alternations dependence). [i]SAP7[/i] can be regulated by the morphogensis’ regulators during [i]C. albicans[/i] growth on epithelium. Adhesion of the mutants was indistinguishable from SC5314. The lack of neither Sap8-10 nor Efg1/Cph1 influences the adhesive behaviour of [i]C. albicans[/i]. Deletion of [i]SAP8-10[/i] resulted in no filamentation defects. The results help understand better the role of [i]SAP7[/i] during adhesion and morphogenesis in [i]C. albicans[/i].
Authors and Affiliations
Monika Staniszewska, Małgorzata Bondaryk, Kamil Żukowski, Michał Chudy
Keywords
Related Articles
- EP ID EP81152
- DOI -
- Views 88
- Downloads 0
Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in Bacteroides Species
We evaluated the usefulness of the Carba NP test for rapid detection of carbapenemase activity in Bacteroides spp. The minimum inhibitory concentration (MIC) for imipenem was determined with gradient test strips, and cfi...
<strong>IgG Avidity: an Important Serologic Marker for the Diagnosis of Tick-Borne Encephalitis Virus Infection</strong>
A total of 52 serum samples from patients with symptoms suggestive of tick-borne encephalitis virus (TBEV) infection and positive IgM and/or IgG antibodies were tested for IgG avidity. Acute/recent TBEV infection was con...
Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer
The traditional CaCO3-based fermentation process generates huge amount of insoluble waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralize...
Activity of Natural Polyether Ionophores: Monensin and Salinomycin against Clinical [i]Staphylococcus epidermidis[/i] Strains
[i]Staphylococcus epidermidis[/i], a coagulase-negative staphylococcus, is the most important pathogen responsible for chronic nosocomial infections. These bacteria produce extracellular slime and form biofilms on v...
CRISPR-Cas Systems in Prokaryotes
Prokaryotic organisms possess numerous strategies that enable survival in hostile conditions. Among others, these conditions include the invasion of foreign nucleic acids such as bacteriophages and plasmids. The clustere...