Role of TaqIA genetic polymorphism of D2 dopamine receptor gene, level of leptin and dopamine in blood in development of various types of obesity in children
Journal Title: Український журнал дитячої ендокринології - Year 2018, Vol 0, Issue 1
Abstract
Objective — to evaluate the role of TaqIA genetic polymorphism of D2 dopamine receptor gene, neuropeptides (leptin, dopamine) level in blood in development of excess body weight in children with various forms of obesity. Materials and methods. We have conducted a crosssectional retrospective study of 288 children aged 0.4—17.9 years, of which 179 are genotyped by TaqIA polymorphic locus (rs1800497) of DRD2 gene. Depending on body mass SDS index the children have been divided into following categories: those with normal body mass (NBM) (–1 to +1; n = 30), those with alimentary (AO) (≥ +2 and < +4; n = 98) and morbid obesity (MO) ((≥ +4; n = 160)). Concentration of dopamine (D) and leptin (L) in blood has been determined by immunosorbent assay method (ELISA). Depending on D level the patients have been divided by quartiles into four groups: 1st group — those with low D level (< 4.99 pg/ml); 2nd group — those with moderately decreased D level (4.99–11.64 pg/ml); 3rd group — those with moderately increased D level (11.65–60.0 pg/ml); 4th group — those with high D level (> 60.0 pg/ml). Similarly, the children have been divided into groups by L concentration: 1st group — those with low L level (< 13.15 ng/ml); 2nd group — those with moderately decreased L level (13.15—23.78 ng/ml); 3rd group — those with moderately increased L level (23.79–40.18 ng/ml); 4th group — those with high L level (> 40.18 ng/ml). Statistical processing has been conducted using the «SPSS Statistics 21» (χ2 criteria and likelihood ratio, р < 0.05). Mathematical modeling method has been applied with the aim of predicting degree of impact of the major factors on development of excess body weight. Results and discussion. In the group with NBM high D level has been identified in 6.7% of children, moderately decreased D level — in 40.0 %, as against the group with MO, in which high D concentration has been identified in 32.0 % of patients, moderately increased D concentration — in 28.0 % (p = 0.038). Differences by L level: low L concentration has been identified in 86.7 % of children with NBM, moderately decreased L concentration — in 34.7 % of patients with AO, high L concentration — in 31.5 % of patients with MO, low L concentration — in 17.7 % (p = 0.0001). A higher A1/A1 genotype frequency by polymorphic locus rs1800497 of DRD2 gene has been identified in children with MO and AO (45.5 % each), as against the group with NBM, in which A1/A1 genotype frequency is statistically significantly lower (9.1 %) (p = 0.012). We have identified no connection between A1/A1 genotype and D and L plasma concentration. We have come up with prognostic models with high statistical estimate (p < 0.05) for determination of degree of probability of development of AO and MO in children based on the presence of А1/А1 genotype of TaqI polymorphic locus of DRD2 gene, D and L level. Conclusions. In patients with AO and MO we have identified predominance of high concentration of D and L compared to patients with NBM. Characteristic for children with NBM is moderately decreased and low level of these neuropeptides (p=0.038; p = 0.0001). It has been determined that А1/А1 genotype of rs1800497 polymorphic locus of DRD2 gene is more common in patients with AO and MO compared to children with NBM (p = 0.012). We have come up with prognostic models with high statistical estimate (p < 0.05) for determination of degree of probability of development of obesity in children based on the presence of А1/А1 genotype of TaqI polymorphic locus of DRD2 gene, D and L level in blood.
Authors and Affiliations
L. S. Viazava, A. V. Solntsava, O. A. Aksionava, O. G. Dashkevich, T. O. Emelyantsava
Keywords
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