RP-HPLC测定糙叶五加不同药用部位中槲皮素和山柰酚
Journal Title: 现代药物与临床 - Year 2011, Vol 26, Issue 4
Abstract
目的 建立同时测定糙叶五加不同药用部位中槲皮素和山柰酚的方法。方法 采用RP-HPLC法，色谱柱为AT.Lichrom ODS-C18柱（250 mm×4.6 mm，5 μm），以甲醇–磷酸–水（50︰0.2︰49.8）为流动相，柱温30 ℃，体积流量1.0 mL/min，检测波长360 nm，进样量10 μL。结果 槲皮素在0.018～0.720 μg线性关系良好（r=0.999 6），加样回收率为98.41%，RSD为0.91%；山柰酚在0.016～0.640 μg线性关系良好（r=0.999 7），加样回收率为98.06%，RSD为1.39%。槲皮素和山柰酚在叶中的量最高，其次是茎，根中未检出。结论 对糙叶五加不同药用部位槲皮素和山柰酚进行了测定，所建立的HPLC法稳定性好、准确性高，适用于糙叶五加中槲皮素和山柰酚的定量分析。
Authors and Affiliations
Yong CHEN , Qin-peng ZOU, Xiang-qian LIU, Jing-ming GAO, O-jin OH
Keywords
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