RP-HPLC-DAD METHOD FOR THE SIMULTANEOUS QUANTIFICATION OF INDACATEROL AND GLYCOPYRRONIUM IN THEIR PHARMACEUTICAL FORMULATION
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2018, Vol 7, Issue 3
Abstract
A simple, sensitive and accurate reversed phase HPLC method was developed and validated for the simultaneous determination of indacaterol maleate and glycopyrronium bromide in commercial powder for inhalation capsules. The method has shown adequate separation of indacaterol maleate and glycopyrronium bromide. Separation was achieved on supelco C18 column (25 cm X 4.6mm, 5μm particle size) using mobile phase consists of acetonitrile: methanol: water (50:30:20, by volume). The retention times for glycopyrronium bromide and indacaterol maleate were 4.051 and 5.310 minutes respectively. Linearity of the proposed method was investigated in the range of 1-12 μg/ml (r2 =0.9998) for indacaterol maleate and 0.5-6 μg/ml (r2 =0.9998) for glycopyrronium bromide. The limit of detection (LOD) was 0.1749 μg/ml for indacaterol maleate and 0.0341μg/ml for glycopyrronium bromide. The limit of quantification (LOQ) was 0.5301 μg/ml for indacaterol maleate and 0.1034 μg/ml for glycopyrronium bromide. The proposed method was validated according to International Conference on Harmonization (ICH) guidelines, statistically compared with a reported spectrophotometric method and successfully applied for the simultaneous determination of both drugs in their combined dosage form.
Authors and Affiliations
Dr. Ayman Osman Elsayed
Keywords
Related Articles
- EP ID EP622150
- DOI -
- Views 133
- Downloads 0
DIFFERENT FORMULATIONS OF PRASRUTA YOGIKA BASTI AND ITS PROBABLE MODE OF ACTION
Basti is one among Panchakarma procedures in Ayurveda. Classically, it is advocated in the diseases of Vata. Prasruta Basti is explained as a separate chapter by Acharya Charaka in Charaka Samhitha Siddi- Sthana 8th chap...
PROTECTIVE EFFECTS OF AQUEOUS STEM EXTRACT OF COSTUS AFER ON HYPERGLYCEAMIC INDUCED HEMATOTOXIC AND HISTOPATHOLOGICAL CHANGES IN WISTAR ALBINO RATS
Aim: Potential protective effects of aqueous stem extract of Costus afer on hyperglyceamically induced heamatotoxic and histopathologic changes were investigated using Wistar albino rat. Method: Thirty matured male albin...
ANTINOCICEPTIVE ACTIVITY EVALUATION OF MOMORDICA CHARANTIA L. FRUIT METHANOLIC EXTRACT
Background. Momordica charantia is a Cucurbitaceae family plant whose fruits are considered to have medicinal values and also consumed as a vegetable. It was of interest to evaluate the antinociceptive activity of methan...
CYTOTOXIC AND WOUND HEALING POTENTIAL OF DMSO EXTRACTS OF PROPOLIS IN MDA-MB-231 CELLS
The aim of this study was to evaluate cytotoxic and wound healing effects of dimethyl sulfoxide (DMSO) extracts of Turkish propolis in MDA-MB-231 cancer cells. For the study of cytotoxic and wound healing effects of prop...
REVIEW ON ANTIDIABETIC DRUGS
Diabetes insipidus is the disorder that causes an imbalance of water in the body. The major complication is the dehydration leads to electrolyte imbalance. Following drugs desmopressin, vasopressin and hydrochlorothazide...