RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OLANZAPINE AND FLUOXETINE IN TABLET DOSAGE FORM
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 4
Abstract
A new precise, accurate, reliable validated method for the determination of Olanzapine and Fluoxetine has been developed by using reverse phase high performance liquid chromatography (RP-HPLC) in pharmaceutical dosage form. Chromatographic separation was carried out by using mobile phase 0.01M Phosphate buffer PH 5.8: Acetonitrile (55:45v/v, PH-2.6 adjusted with Orthophosphoric acid) on HYPERSIL ODS C18 (250 x 4.6 mm, 5) at a flow rate 1ml/min with UV detection at 261nm.The retention times for Olanzapine and Fluoxetine were 3.480 and 2.597 min respectively and both drugs showed good linearity in the range of 18-42μg/ml and 72-168μg/ml. The proposed method has been successfully applied to marketed formulation and was validated according to ICH guidelines and method showed good precision with percentage relative standard deviation less than 2%. The percentage recovery for Olanzapine and Fluoxetine was found between 100.3 and 99.3 respectively indicating the proposed method was accurate and precise.
Authors and Affiliations
V. Pranitha , R. Saraswathi, , Uma Maheshwar Rao V , Ajitha A
Keywords
Related Articles
- EP ID EP122778
- DOI -
- Views 142
- Downloads 0
ASSESSMENT OF HEAVY METAL CONTAMINATION IN PLASTIC MUGS, FREQUENTLY USED AT LOCAL RESTAURANTS OF DIFFERENT DISTRICTS OF U.P. INDIA
A total number of 42 mugs manufactured from plastics (locally made) were purchased and analyzed to determine the level of zinc, nickel, iron and cadmium. All samples were digested with concentrated nitric acid (HNO...
DEVELOPMENT AND VALIDATION OF LORATADINE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY UV SPECTROSCOPIC METHOD
A simple UV spectroscopic method was developed and validated for the estimation of loratadine in bulk and pharmaceutical dosage form using acetonitrile as solvent. The quantification was achieved at 250nm. Beers la...
GREEN ANALYTICAL CHEMISTRY
Analytical methods are developing rapidly, and it is considered to be a small-scale activity, but this is not always true in the case of controlling and monitoring laboratories whose number of runs performed is high. Thi...
ATTENUATED TOTAL REFLECTANCE SPECTROSCOPY: AN OVERVIEW
Attenuated Total Reflectance (ATR) is today the most widely used FTIR sampling tool. ATR generally allows qualitative or quantitative analysis of samples with little or no sample preparation which greatly speeds sample a...
FLASH CHROMATOGRAPHY: AN OVERVIEW
Flash chromatography is a rapid purification technique used to purify compounds of wide ranging polarity; often reaction mixtures, where the target (synthesized) molecule must be separated from excess reagents and reacti...