RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OLANZAPINE AND FLUOXETINE IN TABLET DOSAGE FORM

Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 4

Abstract

A new precise, accurate, reliable validated method for the determination of Olanzapine and Fluoxetine has been developed by using reverse phase high performance liquid chromatography (RP-HPLC) in pharmaceutical dosage form. Chromatographic separation was carried out by using mobile phase 0.01M Phosphate buffer PH 5.8: Acetonitrile (55:45v/v, PH-2.6 adjusted with Orthophosphoric acid) on HYPERSIL ODS C18 (250 x 4.6 mm, 5) at a flow rate 1ml/min with UV detection at 261nm.The retention times for Olanzapine and Fluoxetine were 3.480 and 2.597 min respectively and both drugs showed good linearity in the range of 18-42μg/ml and 72-168μg/ml. The proposed method has been successfully applied to marketed formulation and was validated according to ICH guidelines and method showed good precision with percentage relative standard deviation less than 2%. The percentage recovery for Olanzapine and Fluoxetine was found between 100.3 and 99.3 respectively indicating the proposed method was accurate and precise.

Authors and Affiliations

V. Pranitha , R. Saraswathi, , Uma Maheshwar Rao V , Ajitha A

Keywords

Related Articles

 QUALITATIVE ANALYSIS OF PHYTOCONSTITUENTS IN TRAGIA BREVIPES PLANT

 The study was conducted to analyze the phytoconstituents of Tragia brevipes plant leaves. The plant samples were extracted using methanol and water in the ratio 9:1. The phytoconstituents study was done using stand...

ISOLATION, PURIFICATION AND BIOPHYSICAL CHARACTERIZATION OF BASIC 7S GLOBULIN FROM COCOS NUCIFERA

Elucidation of structure-function relationships of seed storage proteins is a prerequisite for developing theoretically new food and/or food materials based on seed storage proteins. Furthermore, recent findings suggest...

 A VALIDATED SIMULTANEOUS ESTIMATION OF DOXYLAMINE SUCCINATE AND PYRIDOXINE HYDROCHLORIDE BY UV – SPECTROPHOTOMETRIC METHOD IN BULK AND FORMULATION

 A simple and rapid UV – Spectrophotometric method was developed and validated for the simultaneous estimation of Doxylamine succinate and Pyridoxine HCl in combined dosage forms. The said combination is used in tre...

 MUCOADHESIVE VAGINAL DRUG DELIVERY SYSTEM: A REVIEW ON ADVANCE STATUS

 Mucoadhesive delivery systems offer several advantages over other oral controlled release systems by virtue of prolongation of residence time of drug, its targeting, and localization of the dosage form at a specifi...

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION AND STABILITY STUDIES OF THE ESTIMATION OF AGOMELATINE IN TABLET DOSAGE FORM BY RP-HPLC

Agomelatine is a new melatonergic antidepressant with a unique pharmacological action. A stability indicating RP-HPLC method was developed and validated for the determination of agomelatine in active pharmaceutical ingre...

Download PDF file
  • EP ID EP122778
  • DOI -
  • Views 159
  • Downloads 0

How To Cite

V. Pranitha, R. Saraswathi, , Uma Maheshwar Rao V, Ajitha A (2014). RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF OLANZAPINE AND FLUOXETINE IN TABLET DOSAGE FORM. Int J of Pharm Res & Analy, 4(4), 281-284. https://europub.co.uk/articles/-A-122778