RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL AND N-ACETYLCYSTINE IN ITS BULK AND EFFERVESCENT TABLET DOSAGE FORM
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2017, Vol 7, Issue
Abstract
Abstract The present study describes a simple, accurate, precise and cost effective Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method for simultaneous estimation of Paracetamol and N-Acetylcysteine in its bulk and effervescent tablet dosage formulation. The approach of research was to develop an effective RP-HPLC method since there were only few voltammetric methods which were voltammetric in nature. The developed method involved mobile phase containing Water: Acetonitrile in the proportion of 97:3 %V/V, at a flow rate of 1.0 ml/min and oven temperature of 30° C. The analyte were resolved by using isocratic programme on HPLC system containing UV- visible detector with Shimadzu class VP Software and Inertsile C18 (250 mm × 4.6 mm i.d.) 5 μm column. The detection of N-Acetylcysteine and Paracetamol were carried out at 220 nm and the method was validated High linearity of the developed method was confirmed over concentration range of 40-80 μg/ml for Paracetamol and 40-80 μg/ml for N-Acetylcysteine with correlation coefficient of 0.999 and 0.999 respectively at selected wavelength. The percentage RSD for Precision and Accuracy of the method was found to be less than 2%. Peaks were obtained at Retention times of 14.6 & 5.3 min for Paracetamol & N-Acetylcysteine respectively. The major outcomes of the result were that the method showed good reproducibility and it is accurate, precise, specific and sensitive. It can be concluded that the proposed method can be successfully used for the estimation of Paracetamol and N-Acetylcysteine in combination dosage formulations.
Authors and Affiliations
D. Derle, S. Amrutkar*, S. Kulkarni, N. Derle
Keywords
Related Articles
- EP ID EP330591
- DOI -
- Views 131
- Downloads 0
Evaluation of Heteropogon contortus (L.) Beauv. Methanolic extract for Mast cell, Cell Membrane and free radical stabilization
Methanolic extract of Heteropogon contortus (L.) Beauv was screened for mast cell stabilization (in vitro anti-histaminic) and membrane stabilization (in vitro anti-inflammatory) effects. Extracts was also evaluated for...
EVALUATION OF PHYSICAL AND CHEMICAL STABILITY OF DOCETAXEL INCORPORATED NANO STRUCTURED LIPID CARRIER SYSTEM
Abstract Poor physical and chemical stability associated with NLC formulation has been a concern and has taken away the initial attraction in formulating them. Recently it has been reported that the process of lyophiliza...
EXTENDED RELEASE TABLET FORMULATION OF A MACROLIDE ANTIBIOTIC
Abstract Clarithromycin is a macrolide antibiotic. The objective is to design the extended release tablet of Clarithromycin which will exhibit comparative in-vitro and in-vivo drug release profile as that of marketed pro...
PLIGHTS AND PREDICAMENTS IN THE PHARMACY INDUSTRY
We provide an insight to the troubles in the field of pharmacy. Using the published literature to date, we review the impact of various challenges in the field of Pharmacy. The pharmaceutical industry has succumbed to th...
A review: Liposome drug delivery system
Liposomes are the smallest artificial vesicles of spherical shape that can be produced from natural untoxic phospholipids and cholesterol. Liposomes are concentric bilayered vesicles in which aqueous volume is entirely e...