RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS QUANTITATIVE ESTIMATION OF DILOXANIDE FUROATE AND ORNIDAZOLE IN TABLETS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for simultaneous quantitative estimation of Diloxanide furoate and Ornidazole in tablets and validate as per ICH guidelines.Methods: The optimized method uses a reverse phase C18 column, ZODIAC (250 X 4.6 mm; 5μ), mobile phase consisting of mixed phosphate buffer (KH2PO4 and K2HPO4): acetonitrile in the proportion of 30:70 v/v. The mobile phase was set at a flow rate of 1.0 ml/min and the volume injected was 20μl for every injection. The detection wavelength was set at 279 nm. Results: The developed method resulted in Diloxanide furoate eluting at 4.293 min and Ornidazole at 3.34 min. Diloxanide furoate exhibited linearity in the range 90-210μg/ml, while Ornidazole exhibited linearity in the range 60-140μg/ml. The precision is exemplified by relative standard deviations of 0.97% for Diloxanide furoate and 0.3% for Ornidazole. Percentage Mean recoveries were found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) for Diloxanide furoate and Ornidazole were found to be 8.80µg/ml and 6.68µg/ml respectively, while limit of quantitiation (LOQ) for Diloxanide furoate and Ornidazole were found to be 26.6µg/ml and 20.25µg/ml respectively. Conclusion: A simple, accurate, precise, linear and rapid RP-HPLC method was developed for simultaneous quantitative estimation of Diloxanide furoate and Ornidazole in Amicline plus tablets and validated as per ICH guidelines. Hence it can be used for the routine analysis of Diloxanide furoate and Ornidazole in tablets in various pharmaceutical industries.
Authors and Affiliations
P. Sivaprasad, S. Yaswanth Kumar, A. Ashok Kumar
Keywords
Related Articles
- EP ID EP579125
- DOI -
- Views 74
- Downloads 0
STUDY OF PREMENSTRUAL SYNDROME AMONG FUTURE HEALTHCARE PROFESSIONALS IN MASTERSKILL GLOBAL COLLEGE
Objective: Premenstrual syndrome (PMS) is the name given to a collection of physical, psychological and behavioral symptoms that experienced by women during their late luteal phase of each menstrual cycle. This study was...
PHARMACOGNOSTICAL SCREENING AND DETERMINATION OF ANTIOXIDANT ACTIVITY OF NELUMBO NUCIFERA GAERTN ETHANOL SEED EXTRACT BY DIFFERENT IN VITRO MODELS
Objective: To perform the pharmacognostical screening and determination of antioxidant activity of Nelumbo nucifera Gaertn ethanol seed extract by different in vitro models.Methods: The different pharmacognostical parame...
BIOACTIVITY GUIDED FRACTIONATION AND ANTI-INFLAMMATORY ACTIVITY OF ACACIA NILOTICA PODS
Objective: The present study aims at screening the methanol extract and the tannin fraction of the pods of Acacia nilotica for anti-inflammatory activity. Methods: The methanol extract and its tannin fraction were evalua...
INHIBITION OF CANDIDA ALBICANS AND STREPTOCOCCUS MUTANS WITH DATURA LEAF AND SEED EXTRACTS
Objectives: Â Existing antimicrobial compounds are of a limited effectiveness. Therefore, the antimicrobial activity of Datura stramonium extracts was evaluated against two important human pathogens; Candida albicans and...
IMMUNOMODULATORY, ANTICANCEROUS & ANTIOXIDANTACTIVITIES OF CYCLEA PELTATA (LAM.) HOOK.F. & THOMSON
Introduction: Cyclea peltata (Lam.) Hook.f. & Thomson is an important plant listed in Ayurveda. The plants are used in digestive, diuretic and inflammatory disorders. Aim: The present study was to study the immunomo...