RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS QUANTITATIVE ESTIMATION OF METRONIDAZOLE AND NALIDIXIC ACID IN TABLETS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for simultaneous quantitative estimation of Metronidazole and Nalidixic acid in tablets and validate as per ICH guidelines.Methods: The optimized method uses a reverse phase C18 column Inertsil ODS C18 column (250X4.6 mm×5µ) mobile phase consisting of mixed phosphate buffer (pH 4.5; KH2PO4+K2HPO4):methanol: acetonitrile in the proportion of 30:50:20 v/v. The mobile phase was set at a flow rate of 1.2 ml/min and the volume injected was 20μl for every injection. The detection wavelength was set at 271 nm. Results: The developed method resulted in Metronidazole eluting at 2.92 min and Nalidixic acid at 3.99 min. Metronidazole exhibited linearity in the range 30-70μg/ml, while Nalidixic acid exhibited linearity in the range 45-105μg/ml. The precision is exemplified by relative standard deviations of 0.714% for Metronidazole and 0.398% for Nalidixic acid. Percentage Mean recoveries were found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) for Metronidazole and Nalidixic acid were found to be 2.48µg/ml and 2.24µg/ml respectively, while limit of quantitiation (LOQ) for Metronidazole and Nalidixic acid were found to be 7.51µg/ml and 6.79µg/ml respectively.Conclusion: A simple, accurate, precise, linear and rapid RP-HPLC method was developed for simultaneous quantitative estimation of Metronidazole and Nalidixic acid in tablets and validated as per ICH guidelines. Hence it can be used for the routine analysis of Metronidazole and Nalidixic acid in tablets in various pharmaceutical industries.Â
Authors and Affiliations
S. Yaswanth Kumar, P. Sivaprasad, A. Ashok Kumar
Keywords
Related Articles
- EP ID EP579097
- DOI -
- Views 78
- Downloads 0
CHEMOMETRIC SCREENING AND OPTIMIZATION OF LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF SEVEN ANTIHISTAMINES
Objective: Chemometric optimization and validation of a HPLC method for the simultaneous determination of seven antihistamines viz., loratadine, fexofenadine, desloratadine, levocetirizine, doxylamine, promethazine and c...
ANTIDIABETIC STUDY OF COMBINATION OF ANDROGRAPHIS PANICULATA (BURM. F.) WALLICH. EX NEES. HERBS EXTRACT AND GUAZUMA ULMIFOLIA LAMK. LEAVES EXTRACT IN OBESE DIABETIC MICE MODEL
Objective: This research was to investigate the activity of Andrographis paniculata extract (APE) and Guazuma ulmifolia extract (GUE) in treating obesity integrated diabetic conditions.Methods: There were two stages of i...
CYTOTOXIC STUDIES AND PHYTOCHEMICAL ANALYSIS OF ORTHOSIPHON THYMIFLORUS (ROTH) SLEESEN
Objective: Plant-derived compounds with therapeutic effect are getting attention all over the world. Researchers are mainly targeting to ethnic and folk knowledge for the exploration of novel drug candidates. Orthosiphon...
ANTI-ULCER AND ANTIOXIDANT ACTIVITY OF NELUMBO NUCIFERA GAERTN STALKS IN RATS
Objective: Nelumbo nucifera Gaertn. (Nymphaeceae) is a well known aquatic herb which has been extensively used traditionally for the treatment of different diseases. Therefore present study was designed to evaluate the a...
IN VITRO ANTIOXIDANT ACTIVITY OF <em>PROSOPIS CINERARIA</em> LEAVES AGAINST H<sub>2</sub>O<sub>2</sub> INDUCED OXIDATIVE STRESS IN GOAT LIVER SLICES
Objective: To evaluate the antioxidant status of goat liver slices treated with standard oxidant H2O2in the presence and the absence of methanolic leaf extract of Prosopis cineraria.Methods: Precision-cut goat liver slic...