RP-HPLC Method Development and Validation for the Quantitative Estimation of Mirabegron in Extended-Release Tablets
Journal Title: Journal of Medicinal and Chemical Sciences - Year 2018, Vol 1, Issue 2
Abstract
In this study, we describe and validate a rapid and sensitive method for constitution of Mirabegron in the Extended-Release tablets by RP-HPLC. The assay method by HPLC was found to be linear in the concentration range of 10 to 100 µg/mL. Successful separation was achieved by isocratic elution on a Restek C18 column (250 mm × 4.6 mm, 5μm). The mobile phase was composed of buffer pH: 7.0 potassium dihydrogen phosphate and acetonitrile (60:40 v/v) at the flow rate of 1 mL/min using UV detection at 249 nm, column oven temperature 45ºC and injection volume 10 𝜇L. The analytical results were validated by recovery studies. The percentage recovery method was found to be 99.00-101.17%. The LOD and LOQ were found to 0.015 μg/mL and 0.049 μg/mL. All the parameters of validation were in the acceptable range. This developed method was successfully applied for estimate the amount of Mirabegron in the tablets.
Authors and Affiliations
Ali Ramazani, Mehdi Rezaei
Biological and Pharmaceutical Organosulfur Molecules
Organosulfur molecules are prevalent in a broad spectrum of active biological, pharmaceutical and natural molecules. In this category, compounds containing carbon–sulfur bonds occupy a very special place in chemistry sci...
Solvent-Free Acylation of Alcohols, Phenols, Thiols and Amines
The hazardous and toxic nature of many solvents, in particular organic solvents that are extensively used in large scale for organic reactions have transformed a serious threat to the environment. Therefore, the design o...
Photosensitization of phthalocyanine for singlet oxygen generation in photodynamic therapy applications
In this work, phthalocyanine and its OCH3 derivatives were investigated to see their photosensitization characteristics for producing singlet molecular oxygen, which is important in photodynamic therapy type-II. All stru...
A density functional theory study on the effect of size on the ionization potential of different carbon fullerenes
Theoretical investigations were performed to study the structures and properties of different carbon nanoclusters. The computed properties were compared with those of the fullerene. The study systems included carbon full...
Synthesis of icosadeuterio-benzopinacol (Benzopinacol-d20)
This work reports on synthesis of high yield (92%) of isosadeuterio-benzopinacol (benzopinacol-d20) via photo-reductive dimerization of benzophenone-d10. The latter compound was obtained using an improved method to achie...