RP-HPLC Method development and validation for the simultaneous estimation of Aceclofenac and Rabeprazole Sodium in the bulk and marketed formulation
Journal Title: Indian Journal of Pharmacy and Pharmacology - Year 2016, Vol 3, Issue 3
Abstract
A simple, accurate and precise RP-HPLC method was developed for the estimation of Aceclofenac and Rabeprazole Sodium in the bulk and marketed formulation. The method was performed by using the mobile phase containing Methnol, Water and Acetonitrile (60:30:10 v/v), pH- 6.14 was found to be most suitable for RP-HPLC as it showed sharp peak with symmetry and significant reproducible retention time for Aceclofenac (4.549 min) along with Rabeprazole Sodium (6.048 min). From the results of system suitability parameters, it was observed that the peak was sharp and symmetrical with satisfactory capacity factor and column efficiency. From the developed methods, percent content of Aceclofenac and Rabeprazole Sodium was found to be 99.97%, 99.91% and 99.94% 99.6%, 99.8% and 94.45% respectively by RP-HPLC. The method was validated by parameters like accuracy, precision, specificity, linearity, ruggedness and robustness. The results indicated that, the method is simple, accurate, precise, rugged. The method described enables to the quantification of Aceclofenac and Rabeprazole Sodium. The advantages lie in the simplicity of sample preparation and the low costs of reagents used. The proposed HPLC conditions ensure sufficient resolution and the precise quantification of the compounds. Results from statistical analysis of the experimental results were indicative of satisfactory precision and reproducibility. Hence, this HPLC method can be used for routine drug analysis
Authors and Affiliations
Vaibhav S. Adhao
Keywords
Related Articles
- EP ID EP240687
- DOI -
- Views 129
- Downloads 0
Steven-Johnson Syndrome following Sodium Valproate Monotherapy
Steven-Johnson Syndrome (SJS) is a severe, episodic, acute mucocutaneous reaction characterized by blisters and epidermal detachment resulting from epidermal necrosis in the absence of substantial dermal inflammation. We...
Evaluation and comparison of hypoglycemic activity of bark and leaf of Ficus bengalensis linn. in alloxan induced diabetes in albino rats
Objectives: Evaluation of Hypoglycemic activity of Extract of bark and leaf of Ficus bengalensis Linn.in alloxan induced diabetic albino rats and comparison with standard antihyperglycemic drug Glibenclamide. Materials a...
Attenuation of Sub Acute Inflammation by Ethanol Extract of Clerodendrum viscosum Linn. Leaves in Wistar Albino Rats
Inflammation is a pathological response of vascular tissue for harmful stimuli caused by injury, infection, environmental agents, malignancy and cellular changes. In our study we have tried to evaluate attenuation effect...
Assessment of knowledge of medical post graduate students regarding promotional drug literature using WHO criteria
Objective Pharmaceutical promotional activities create potential for inappropriate prescribing practices without necessarily benefiting the patients but contributes to increased health care costs if prescriber is not awa...
Evaluation of antidepressant effects of rosuvastatin in male albino mice by tail suspension model
Objective: To evaluate the antidepressant activity of Rosuvastatin in male albino mice. Method: Thirty male swiss albino mice were selected for tail suspension model. There were five groups and each group had six animals...