RP-HPLC method development and validation for the simultaneous estimation of Miconazole and Clindamycin in pharmaceutical dosage forms
Journal Title: Scholars Academic Journal of Pharmacy - Year 2017, Vol 6, Issue 1
Abstract
Abstract:A simple, Accurate, precise method was developed for the simultaneous estimation of the Miconazole and Clindamycin in Tablet dosage form by RP-HPLC. Chromatogram was run through kromasil 150mm x 4.6 mm, 5m. Mobile phase containing Buffer and Acetonitrile taken in the ratio 20:80 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.01N KH2PO4solution. Temperature was maintained at 30°C. Optimized wavelength for Miconazole and Clindamycin was 210nm. Retention time of Miconazole and Clindamycin were found to be 2.076min and 2.795min. % RSD of the Miconazole and Clindamycin were and found to be 0.99 and 1.05 respectively. Percentage assay was obtained as 100.23% and 99.25% for Miconazole and Clindamycin respectively. LOD, LOQ values are obtained from regression equations of Miconazole and Clindamycin were 1.42ppm, 0.60ppm and 4.31pm, 1.82ppm respectively. Regression equation of Miconazole is y = 19688x + 25044, and y = 18253x + 2735.5 of Clindamycin. Keywords:Miconazole, Clindamycin, RP-HPLC, Retention time, Assay.
Authors and Affiliations
Ganesh Akula, Vanamdas Saibabu, S. S. Phanindra, Rangu Nirmal, Santhosh Suddagoni, Dr. A. Jaswanth
Keywords
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