RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF SEXAGLIPTIN AND PIOGLITAZONE IN TABLETS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 5
Abstract
A simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the analysis of Sexagliptin and Pioglitazone. Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5m , 150 mm x 4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (60:40, v/v, pH 7.0) at a flow rate of 0.8 ml/min with UV detection at 260nm.Aceclofenac was used as an internal standard. The retention time of Sexagliptin, pioglitazone and aceclofenac was 2.48, 4.45 and 6.34 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation.This study aimed at developing and validating an HPLC method, being simple, accurate and selective, and the proposed method can be used for the estimation of these drugs in combined dosage forms.
Authors and Affiliations
M. Sarat , P. Murali krishna , C Rambabu
Keywords
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