S0Synergistic effect of selected anticancer drugs with Vitamin – E against HT29 human adenocarcinoma cell lines
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2014, Vol 2, Issue 4
Abstract
The present study was investigate to study the synergistic effects of selected anti-cancer drugs i.e. 5-FU, IR and Vitamin-E (Vit-E) on induction of apoptosis in HT29 cells. GI50 values were determined by MTT assay and was found to be 25, 25μM for the individual compounds and it was found that at 200μM higher concentration of Vit-E has nosignificant cytotoxicity with HT29 cells. On combination of anticancer drugs with Vit-E has significantly reduced the drug dose with the higher cytotoxic efficacy. The light microscopic image revealed that on treatment with individual and combination of anticancer drug with Vit-E showed majority of HT29 cells had shrunken and rounded up from their culture surface when compared with control cells. Further the DNA ladder assay confirms the individual and combination of anticancer drug and Vit-E induced apoptotic genomic DNA fragmentation. In regard to kinetics of apoptotic cells induced by treatment, the maximum induction of apoptosis by the combined treatment with vit E was higher than that of individual anticancer drugs was proved using confocal microscopy bydual staining with AO/EB. Present study implicit the observation that Vit-E has promising anticancer ingredients in combination with IR and 5-FU. Moreover our findings demonstrate that incubation of anticancer drugs with Vit E resulted in significant cell growth inhibition, DNA fragmentation. Apparently, the promising active principles and underlying mechanism by which this activity was exhibited need to be further investigated.
Authors and Affiliations
Karthi Natesan, Manju Vaiyapuri
Keywords
Related Articles
- EP ID EP33241
- DOI -
- Views 268
- Downloads 0
Toxicity studies of combined extracts of Vitex pubescence, Vitex penducularis and Vitex agnuscastus
For the acute study, Combined extracts of Vitex pubescence, Vitex penducularis and Vitex agnuscastus was administrated to mice in single doses given by oral route. General behavior, adverse effects and mortality were d...
Analytical method development and validation for the simultaneous estimation of Paracetamol and Tapentadol by RP-HPLC in bulk and pharmaceutical dosage forms
A simple rapid, accurate, precise and reproducible validated reverse phase HPLC method was developed for the determination of Paracetamol and Tapentadol in bulk and pharmaceutical dosage forms. The quantification was c...
SYNTHESIS OF NEW THIAZOLIDINE-2,4-DIONE DERIVATIVES AND THEIR ANTIMICROBIAL AND ANTITUBERCULAR ACTIVITY
New 1,3-thiazolidine-2,4-dione (TZD) derivatives 16-29 have been prepared by Knoevenagel condensation reaction between TZD and aromatic aldehydes followed by condensation with 3,4- dichloro benzoyl chloride. The struct...
Analytical method development for the simultaneous estimation of Hydrochlorothiazide and Atenolol in combined tablet formulation by RP-HPLC
A Simple, accurate, rapid, sensitive and precise Rp-Hplc method has been developed for estimation of Hydrochlorothiazide and Atenolol in tablet dosage form using PDA detector. A Reverse phase Thermo Hypersil ODS c18 co...
Formulation and evaluation sustained release matrix tablets of Propranol hydrochloride
Propranolol hydrochloride is the most recently a non-selective beta-adrenergic blocking agent, has been widely used in the treatment of hypertension, angina pectoris, phaeochromocytoma, cardiac arrhythmias and other ca...